Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
Autor/a
Riera-Heredia, Natàlia
Martins, Rute
Mateus, Ana Patrícia
Costa, Rita A.
Navarro, Isabel
Gutiérrez, Joaquim
Power, Deborah M.
Capilla, Encarnación
Fecha de publicación
2018-07-25ISSN
2045-2322
Resumen
This study aimed to characterize the molecules involved in osteogenesis in seabream and establish using in vitro/in vivo approaches the responsiveness of selected key genes to temperature. The impact of a temperature drop from 23 to 13 °C was evaluated in juvenile fish thermally imprinted during embryogenesis. Both, in vitro/in vivo, Fib1a, appeared important in the first stages of bone formation, and Col1A1, ON and OP, in regulating matrix production and mineralization. OCN mRNA levels were up-regulated in the final larval stages when mineralization was more intense. Moreover, temperature-dependent differential gene expression was observed, with lower transcript levels in the larvae at 18 °C relative to those at 22 °C, suggesting bone formation was enhanced in the latter group. Results revealed that thermal imprinting affected the long-term regulation of osteogenesis. Specifically, juveniles under the low and low-to-high-temperature regimes had reduced levels of OCN when challenged, indicative of impaired bone development. In contrast, gene expression in fish from the high and high-to-low-temperature treatments was unchanged, suggesting imprinting may have a protective effect. Overall, the present study revealed that thermal imprinting modulates bone development in seabream larvae, and demonstrated the utility of the in vitro MSC culture as a reliable tool to investigate fish osteogenesis.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
639 - Caza. Pesca. Piscicultura
Páginas
14
Publicado por
Springer Nature
Publicado en
Scientific Reports
Citación
Riera-Heredia, Natàlia, Rute Martins, Ana Patrícia Mateus, Rita A. Costa, Enric Gisbert, Isabel Navarro, Joaquim Gutiérrez, Deborah M. Power, and Encarnación Capilla. 2018. "Temperature Responsiveness Of Gilthead Sea Bream Bone; An In Vitro And In Vivo Approach". Scientific Reports 8 (1). Springer Nature. doi:10.1038/s41598-018-29570-9.
Número del acuerdo de la subvención
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-17324/ES/CARACTERIZACION DE MOLECULAS CLAVE INVOLUCRADAS EN LA OSTEOBLASTOGENESIS, EL DESARROLLO OSEO Y LA APARICION DE DEFORMIDADES EN LA DORADA (SPARUS AURATA)/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-57974-R/ES/ADIPOSIDAD Y METABOLISMO LIPIDICO EN PECES ALIMENTADOS CON DIETAS DE ACEITE DE PESCADO ALTAMENTE SUBSTITUIDO Y CRIADOS A ELEVADAS TEMPERATURAS: ENFOQUES IN VITRO E IN VIVO/
EC/FP7/222719/EU/Building a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture/LIFECYCLE
Program
Aqüicultura
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