Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR
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Author
Macedo, Nubia
Gottschalk, Marcelo
Strutzberg-Minder, Katrin
Van, Chao Nguyen
Zhang, Lijun
Zou, Geng
Zhou, Rui
Marostica, Thaire
Clavijo, Maria Jose
Tucker, Alexander
Publication date
2021-05-12ISSN
0928-4249
Abstract
Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, and 13 were the most commonly detected. Serovars of greatest clinical relevance were systemic isolates that had a higher probability of being serovar 5/12, 13, or 7. In comparison, pulmonary isolates had a higher likelihood of being serovars 2, 4, 7, or 14. Serovars 5/12 and 13 have previously been considered disease-associated, but this study agrees with other recent studies showing that serovar 7 is indeed associated with systemic G. parasuis disease. Serovar 4 strains illustrated how isolates can have varying degrees of virulence and be obtained from pulmonary, systemic, or nasal sites. Serovars 8, 9, 15, and 10 were predominantly obtained from nasal samples, which indicates a limited clinical significance of these serovars. Additionally, most internal G. parasuis isolates were classified as virulent by LS-PCR and were disease-associated isolates, including serovars 1, 2, 4, 5/12, 7, 13, and 14. Isolates from the nasal cavity, including serovars 6, 9, 10, 11, and 15, were classified as non-virulent by LS-PCR. In conclusion, the distribution of G. parasuis serovars remains constant, with few serovars representing most of the strains isolated from affected pigs. Moreover, it was confirmed that the LS-PCR can be used for G. parasuis virulence prediction of field strains worldwide.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
10
Publisher
BMC
Is part of
Veterinary Research
Citation
Macedo, Nubia, Marcelo Gottschalk, Katrin Strutzberg-Minder, Chao Nguyen Van, Lijun Zhang, Geng Zou, and Rui Zhou et al. 2021. "Molecular Characterization Of Glaesserella Parasuis Strains Isolated From North America, Europe And Asia By Serotyping PCR And LS-PCR". Veterinary Research 52 (1). doi:10.1186/s13567-021-00935-9.
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2651]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/