Amplified plasmonic and microfluidic setup for DNA monitoring
Guerreiro, J.Rafaela L.
Data de publicació2021-09-07
Plasmonic nanosensors for label-free detection of DNA require excellent sensing resolution, which is crucial when monitoring short DNA sequences, as these induce tiny peak shifts, compared to large biomolecules. We report a versatile and simple strategy for plasmonic sensor signal enhancement by assembling multiple (four) plasmonic sensors in series. This approach provided a fourfold signal enhancement, increased signal-to-noise ratio, and improved sensitivity for DNA detection. The response of multiple sensors based on AuNSpheres was also compared with AuNRods, the latter showing better sensing resolution. The amplification system based on AuNR was integrated into a microfluidic sequential injection platform and applied to the monitoring of DNA, specifically from environmental invasive species—zebra mussels. DNA from zebra mussels was log concentration-dependent from 1 to 1 × 106 pM, reaching a detection limit of 2.0 pM. In situ tests were also successfully applied to real samples, within less than 45 min, using DNA extracted from zebra mussel meat. The plasmonic nanosensors’ signal can be used as a binary output (yes/no) to assess the presence of those invasive species. Even though these genosensors were applied to the monitoring of DNA in environmental samples, they potentially offer advantage in a wide range of fields, such as disease diagnostics.
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Versió del document
574 - Ecologia general i biodiversitat
Guerreiro, J. Rafaela L., Andrey Ipatov, Joana Carvalho, Anna Toldrà, and Marta Prado. 2021. "Amplified Plasmonic And Microfluidic Setup For DNA Monitoring". Microchimica Acta 188 (10). doi:10.1007/s00604-021-04983-y.
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