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dc.contributor.authorShkembi, Xhensila
dc.contributor.authorSkouridou, Vasso
dc.contributor.authorSvobodova, Marketa
dc.contributor.authorLeonardo, Sandra
dc.contributor.authorBashammakh, Abdulaziz S.
dc.contributor.authorAlyoubi, Abdulrahman O.
dc.contributor.authorCampàs, Mònica
dc.contributor.authorO'Sullivan, Ciara K.
dc.contributor.otherProducció Animalca
dc.date.accessioned2022-01-18T13:50:54Z
dc.date.available2022-01-18T13:50:54Z
dc.date.issued2021-10-26
dc.identifier.citationShkembi, Xhensila, Vasso Skouridou, Marketa Svobodova, Sandra Leonardo, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Mònica Campàs, and Ciara K. O′Sullivan. 2021. “Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish”. Analytical Chemistry 93 (44): 14810–14819. doi.org/10.1021/acs.analchem.1c03671.ca
dc.identifier.issn0003-2700ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1468
dc.description.abstractThe marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a highly sensitive and user-friendly hybrid antibody–aptamer sandwich assay was developed with superior performance compared to several assays reported in the literature and commercial immunoassay kits. The assay was successfully applied to the quantification of TTX in pufferfish extracts, and the results obtained correlated very well with a competitive magnetic bead-based immunoassay performed in parallel for comparison. This is one of the very few works reported in the literature of such hybrid assays for small-molecule analytes whose compatibility with field samples is also demonstrated.ca
dc.format.extent10ca
dc.language.isoengca
dc.publisherAmerican Chemical Societyca
dc.relation.ispartofAnalytical Chemistryca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleHybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfishca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMICINN-AEI/Programas Estatales de generación de conocimiento y fortalecimiento científico y tecnológico del sistema de I+D+i y de I+D+i orientada a los retos de la sociedad, convocatoria 2020/PID2020-112976RB-C21/ES/Biotechnological tools based on cells and aptamers for the detection of ciguatoxins and tetrodotoxins/CELLECTRAca
dc.relation.projectIDMICINN-AEI/Programas Estatales de generación de conocimiento y fortalecimiento científico y tecnológico del sistema de I+D+i y de I+D+i orientada a los retos de la sociedad, convocatoria 2020/PID2020-112976RB-C22/ES/Biotechnological tools based on cells and aptamers for the detection of ciguatoxins and tetrodotoxins/CELLECTRAca
dc.subject.udc574ca
dc.identifier.doihttps://doi.org/10.1021/acs.analchem.1c03671ca
dc.contributor.groupAigües Marines i Continentalsca


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