Understanding host-microbiota interactions in the commercial piglet around weaning

Abstract

Weaning is a critical period in the life of pigs with repercussions on their health and welfare and on the economy of the swine industry. This study aimed to assess the efect of the commercial early weaning on gut microbiota, intestinal gene expression and serum metabolomic response via an integratedomic approach combining 16S rRNA gene sequencing, the OpenArray gene expression technology and 1 H-NMR spectroscopy. Fourteen piglets from diferent litters were sampled for blood, jejunum tissue and caecal content two days before (− 2d), and three days after (+3d) weaning. A clearly diferential ordination of caecal microbiota was observed. Higher abundances of Roseburia, Ruminococcus, Coprococcus, Dorea and Lachnospira genera in weaned piglets compared to prior to weaning showed the quick microbial changes of the piglets’ gut microbiota. Downregulation of OCLN, CLDN4, MUC2, MUC13, SLC15A1 and SLC13A1 genes, also evidenced the negative impact of weaning on gut barrier and digestive functions. Metabolomic approach pinpointed signifcant decreases in choline, LDL, triglycerides, fatty acids, alanine and isoleucine and increases in 3-hydroxybutyrate after weaning. Moreover, the correlation between microbiota and metabolome datasets revealed the existence of metabolic clusters interrelated to diferent bacterial clusters. Our results demonstrate the impact of weaning stress on the piglet and give insights regarding the associations between gut microbiota and the animal gene activity and metabolic response.