Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs
Bohórquez, José Alejandro
Gladue, Douglas P.
Borca, Manuel V.
Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate.
619 - Veterinària
Is part of
Bohórquez, José A., Sira Defaus, Rosa Rosell, Marta Pérez-Simó, Mònica Alberch, Douglas P. Gladue, Manuel V. Borca, David Andreu, and Llilianne Ganges. 2021. "Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs" Viruses 13, no. 10: 1980. https://doi.org/10.3390/v13101980
Grant agreement number
MINECO/Programa estatal de I+D+I orientada a los retos de la sociedad/RTI2018-100887-B-100/ES/Descifrando nuevos factores virales y del hospedador involucrados en el desarrollo de la peste porcina clásica: implicaciones para el control de la enfermedad/
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS 
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/