Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
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Autor/a
Marín, Belén
Otero, Alicia
Lugan, Séverine
Espinosa, Juan Carlos
Marín-Moreno, Alba
Hedman, Carlos
Romero, Antonio
Pumarola, Martí
Badiola, Juan J.
Torres, Juan María
Andréoletti, Olivier
Bolea, Rosa
Fecha de publicación
2021-08-31ISSN
2045-2322
Resumen
Pigs are susceptible to infection with the classical bovine spongiform encephalopathy (C-BSE) agent following experimental inoculation, and PrPSc accumulation was detected in porcine tissues after the inoculation of certain scrapie and chronic wasting disease isolates. However, a robust transmission barrier has been described in this species and, although they were exposed to C-BSE agent in many European countries, no cases of natural transmissible spongiform encephalopathies (TSE) infections have been reported in pigs. Transmission of atypical scrapie to bovinized mice resulted in the emergence of C-BSE prions. Here, we conducted a study to determine if pigs are susceptible to atypical scrapie. To this end, 12, 8-9-month-old minipigs were intracerebrally inoculated with two atypical scrapie sources. Animals were euthanized between 22- and 72-months post inoculation without clinical signs of TSE. All pigs tested negative for PrPSc accumulation by enzyme immunoassay, immunohistochemistry, western blotting and bioassay in porcine PrP mice. Surprisingly, in vitro protein misfolding cyclic amplification demonstrated the presence of C-BSE prions in different brain areas from seven pigs inoculated with both atypical scrapie isolates. Our results suggest that pigs exposed to atypical scrapie prions could become a reservoir for C-BSE and corroborate that C-BSE prions emerge during interspecies passage of atypical scrapie.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
619 - Veterinaria
Páginas
8
Publicado por
Nature Research
Publicado en
Scientific Reports
Citación
Marín, Belén, Alicia Otero, Séverine Lugan, Juan Carlos Espinosa, Alba Marín-Moreno, Enric Vidal, and Carlos Hedman et al. 2021. "Classical BSE Prions Emerge From Asymptomatic Pigs Challenged With Atypical/Nor98 Scrapie". Scientific Reports 11 (1). doi:10.1038/s41598-021-96818-2.
Número del acuerdo de la subvención
EC/INTERREG-POCTEFA/EU/EFA-148-16/ /REDPRION
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/PID2019-105837RB-I00/ES/EVOLUCION A TRAVES DE LA BARRERA DE ESPECIE DEL POTECIAL ZOONOTICO DE LOS PRIONES EMERGENTES/
Program
Sanitat Animal
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