A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
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Autor/a
López-Girona, Elena
Zhang, Yu
Hernández Mora, José Ramón
Alexiou, Konstantinos G.
Arús, Pere
Aranzana, María José
Fecha de publicación
2017-07-27ISSN
2045-2322
Resumen
In peach, the fat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from
homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identifed a SSR
marker (UDP98–412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion afecting the gene PRUPE.6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRRRLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group. PCR markers suitable for MAS confrmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the fat variety ‘UFO-4’, revealing that the mutation afected the fat associated allele (S). Protein BLAST alignment identifed signifcant hits with genes involved in diferent biological processes. Best protein hit occurred with AtRLP12, which may functionally complement CLAVATA2, a key regulator that controls the stem cell population size. RT-PCR analysis revealed the absence of transcription of the partially deleted allele. The data support PRUPE.6G281100 as a candidate gene for fat shape in peach.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
633 - Cultivos y producciones
Páginas
11
Publicado por
Nature Research
Publicado en
Scientific Reports
Citación
López-Girona, Elena, Yu Zhang, Iban Eduardo, José Ramón Hernández Mora, Konstantinos G. Alexiou, Pere Arús, and María José Aranzana. 2017. "A Deletion Affecting An LRR-RLK Gene Co-Segregates With The Fruit Flat Shape Trait In Peach". Scientific Reports 7 (1). doi:10.1038/s41598-017-07022-0.
Número del acuerdo de la subvención
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-68329-R/ES/IDENTIFICACION Y CARACTERIZACION DE GENES IMPLICADOS EN LA FORMA Y JUGOSIDAD DEL FRUTO EN MELOCOTONERO Y ALMENDRO/
Program
Genòmica i Biotecnologia
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