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Cell immunolocalization of ciguatoxin-like compounds in the benthic dinoflagellate Gambierdiscus australes M. Chinain & M.A. Faust by confocal microscopy
dc.contributor.author | Honsell, Giorgio | |
dc.contributor.author | Gaiani, Greta | |
dc.contributor.author | Hirama, Masahiro | |
dc.contributor.author | Pelin, Marco | |
dc.contributor.author | Tubaro, Aurelia | |
dc.contributor.author | Tsumuraya, Takeshi | |
dc.contributor.author | Campàs, Mònica | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2023-02-08T13:37:23Z | |
dc.date.issued | 2022-11-23 | |
dc.identifier.citation | Honsell, Giorgio, Greta Gaiani, Masahiro Hirama, Marco Pelin, Aurelia Tubaro, Takeshi Tsumuraya, and Mònica Campàs. 2022. "Cell Immunolocalization Of Ciguatoxin-Like Compounds In The Benthic Dinoflagellate Gambierdiscus Australes M. Chinain &Amp; M.A. Faust By Confocal Microscopy". Harmful Algae 120: 102353. doi:10.1016/j.hal.2022.102353. | ca |
dc.identifier.issn | 1568-9883 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/2035 | |
dc.description.abstract | Dinoflagellates of the genera Gambierdiscus and Fukuyoa are able to produce potent neurotoxins like ciguatoxins (CTXs), which, after biooxidation in fish, are responsible for ciguatera intoxication. An isolate of G. australes from the Canary Islands, that revealed the presence of CTX-like compounds by immunosensing tools, was studied by immunocytochemistry to localize intracellular CTX-like compounds, using 8H4 monoclonal antibody that specifically recognizes the right wing of CTX1B and CTX3C analogues. Confocal microscopy observations of immunostained whole cells revealed a strong positive reaction on cell surface and all along the cell outline, while no reaction was detected inside the cells, probably because the antibody was not able to pass through thecal plates. Cell sections showed a positive antibody staining not only on thecal plates, but also inside cytoplasm, with numerous small dots and larger tubule-like reticulate structures. Small fluorescent dots were detected also on the nuclear surface. These observations indicate that CTX-like compounds are present in G. australes cytoplasm, and then are, at least in part, released to cover the cell surface. | ca |
dc.format.extent | 25 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | Harmful Algae | ca |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Cell immunolocalization of ciguatoxin-like compounds in the benthic dinoflagellate Gambierdiscus australes M. Chinain & M.A. Faust by confocal microscopy | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/embargoedAccess | |
dc.date.embargoEnd | 2024-11-22T01:00:00Z | |
dc.embargo.terms | 24 mesos | ca |
dc.relation.projectID | EC/H2020/778069/EU/Emergent Marine Toxins in the North Atlantic and Mediterranean: New Approaches to Assess their Occurrence and Future Scenarios in the Framework of Global Environmental Changes/EMERTOX | ca |
dc.relation.projectID | MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA | ca |
dc.subject.udc | 574 | ca |
dc.identifier.doi | https://doi.org/10.1016/j.hal.2022.102353 | ca |
dc.contributor.group | Aigües Marines i Continentals | ca |
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