An LTR retrotransposon in the promoter of a PsMYB10.2 gene associated with the regulation of fruit f lesh color in Japanese plum
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Author
Fiol, Arnau
García, Sergio
Dujak, Christian
Pacheco, Igor
Infante, Rodrigo
Publication date
2022-09-13ISSN
2052-7276
Abstract
Japanese plums exhibit wide diversity of fruit coloration. The red to black hues are caused by the accumulation of anthocyanins, while their absence results in yellow, orange or green fruits. In Prunus, MYB10 genes are determinants for anthocyanin accumulation. In peach, QTLs for red plant organ traits map in an LG3 region with three MYB10 copies (PpMYB10.1, PpMYB10.2 and PpMYB10.3). In Japanese plum the gene copy number in this region differs with respect to peach: there are at least three copies of PsMYB10.1, with the expression of one of them (PsMYB10.1a) correlating with fruit skin color. The objective of this study was to determine a possible role of LG3-PsMYB10 genes in the natural variability of the f lesh color trait and to develop a molecular marker for marker-assisted selection (MAS). We
explored the variability within the LG3-PsMYB10 region using long-range sequences obtained in previous studies through CRISPR-Cas9 enrichment sequencing. We found that the PsMYB10.2 gene was only expressed in red f lesh fruits. Its role in promoting anthocyanin biosynthesis was validated by transient overexpression in Japanese plum fruits. The analysis of long-range sequences identified an LTR retrotransposon in the promoter of the expressed PsMYB10.2 gene that explained the trait in 93.1% of the 145 individuals analyzed. We hypothesize that the LTR retrotransposon may promote the PsMYB10.2 expression and activate the anthocyanin biosynthesis pathway. We propose for the first time the PsMYB10.2 gene as candidate for the f lesh color natural variation in Japanese plum and provide a
molecular marker for MAS.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
633 - Cultius i produccions
Pages
12
Publisher
Oxford University Press
Is part of
Horticulture Research
Citation
Fiol, Arnau, Sergio García, Christian Dujak, Igor Pacheco, Rodrigo Infante, and Maria José Aranzana. 2022. "An LTR Retrotransposon In The Promoter Of A Psmyb10.2 Gene Associated With The Regulation Of Fruit Flesh Color In Japanese Plum". Horticulture Research 9. doi:10.1093/hr/uhac206.
Grant agreement number
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-100795-B-I00/ES/HERRAMIENTAS MOLECULARES PARA LA MEJORA GENETICA DE CARACTERES DE CALIDAD EN MELOCOTONERO Y OTROS PRUNUS/
MC/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-128885OB-I00/ES/ /
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ /
FEDER/ / /EU/ /
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2054]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/