A new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates
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Autor/a
Gifre-Renom, L.
Cano-Garrido, O.
Fàbregas, F.
Roca-Pinilla, R.
Seras-Franzoso, J.
Ferrer-Miralles, N.
Villaverde, A.
Bach, A.
Devant, M.
Arís, A.
Garcia-Fruitós, E.
Fecha de publicación
2018-09-17ISSN
2045-2322
Resumen
The production of pure and soluble proteins is a complex, protein-dependent and time-consuming
process, in particular for those prone-to-aggregate and/or difcult-to-purify. Although Escherichia coli is
widely used for protein production, recombinant products must be co-purifed through costly processes
to remove lipopolysaccharide (LPS) and minimize adverse efects in the target organism. Interestingly,
Lactococcus lactis, which does not contain LPS, could be a promising alternative for the production
of relevant proteins. However, to date, there is no universal strategy to produce and purify any
recombinant protein, being still a protein-specifc process. In this context and considering that L. lactis
is also able to form functional protein aggregates under overproduction conditions, we explored the use
of these aggregates as an alternative source of soluble proteins. In this study, we developed a widely
applicable and economically afordable protocol to extract functional proteins from these nanoclusters.
For that, two model proteins were used: mammary serum amyloid A3 (M-SAA3) and metalloproteinase
9 (MMP-9), a difcult-to-purify and a prone-to-aggregate protein, respectively. The results show that
it is possible to obtain highly pure, soluble, LPS-free and active recombinant proteins from L. lactis
aggregates through a cost-efective and simple protocol with special relevance for difcult-to-purify or
highly aggregated proteins.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
636 - Explotación y cría de animales. Cría del ganado y de animales domésticos
Páginas
10
Publicado por
Nature Research
Publicado en
Scientific Reports
Citación
Gifre-Renom, L., O. Cano-Garrido, F. Fàbregas, R. Roca-Pinilla, J. Seras-Franzoso, N. Ferrer-Miralles, and A. Villaverde et al. 2018. "A New Approach To Obtain Pure And Active Proteins From Lactococcus Lactis Protein Aggregates". Scientific Reports 8 (1). Springer Nature America, Inc. doi:10.1038/s41598-018-32213-8.
Número del acuerdo de la subvención
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00064-C02-01/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00064-C02-02/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/
Program
Producció de Remugants
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