A chromosome-level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex determining gene in the flatfish Solea senegalensis
Author
de la Herrán, Roberto
Hermida, Miguel
Rubiolo, Juan Andres
Gómez-Garrido, Jèssica
Cruz, Fernando
Robles, Francisca
Navajas-Pérez, Rafael
Blanco, Andres
Rodriguez Villamayor, Paula
Torres, Dorinda
Sánchez-Quinteiro, Pablo
Ramirez, Daniel
Rodríguez, Maria Esther
Arias-Pérez, Alberto
Cross, Ismael
Martínez-Peña, Teresa
Riaza, Ana
Millán, Adrian
De Rosa, M.Cristina
Pirolli, Davide
Gut, Marta
Bouza, Carmen
Robledo, Diego
Rebordinos, Laureana
Alioto, Tyler
Ruíz-Rejón, Carmelo
Martínez, Paulino
Publication date
2023-01-01ISSN
1755-098X
Abstract
Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 SNP variants in the follicle stimulating hormone receptor (fshr) consistent with an XX / XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. Fshr displayed differential gene expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 non-synonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
637 - Produce of domestic (farmyard) animals and game
Pages
19
Publisher
Wiley
Is part of
Molecular Ecology Reseources
Citation
de la Herrán, Roberto, Miguel Hermida, Juan Andres Rubiolo, Jèssica Gómez-Garrido, Fernando Cruz, Francisca Robles, Rafael Navajas-Pérez, et al. "A Chromosome-Level Genome Assembly Enables the Identification of the Follicule Stimulating Hormone Receptor as the Master Sex-Determining Gene in the Flatfish Solea Senegalensis." Molecular Ecology Resources . doi:10.1111/1755-0998.13750
Grant agreement number
MICIU/Programa Estatal de I+D+i orientada a los retos de la sociedad/RTI2018-096847-B-C21/ES/Estudios genómicos y de determinación sexual en el lenguado (Solea senegalensis) mediante integración de mapas/
MICIU/Programa Estatal de I+D+i orientada a los retos de la sociedad/RTI2018-096847-B-C22/ES/Desarrollo del mapa de ligamento diploide del lenguado y caracterización de secuencias repetidas y genes con expresión diferencial entre sexos/
MICIU/Programa Estatal de I+D+i orientada a los retos de la sociedad/RTI2018-097110-B-C21/ES/Análisis de crecimiento y anomalías vertebrales en lenguado senegalés (Solea senegalensis): búsqueda de biomarcadores de calidad y bienestar en piscicultura/
EC/H2020/817923/EU/Advancing European Aquaculture by Genome Functional Annotation/AQUA-FAANG
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2549]
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/