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dc.contributor.authorde la Herrán, Roberto
dc.contributor.authorHermida, Miguel
dc.contributor.authorRubiolo, Juan Andres
dc.contributor.authorGómez-Garrido, Jèssica
dc.contributor.authorCruz, Fernando
dc.contributor.authorRobles, Francisca
dc.contributor.authorNavajas-Pérez, Rafael
dc.contributor.authorBlanco, Andres
dc.contributor.authorRodriguez Villamayor, Paula
dc.contributor.authorTorres, Dorinda
dc.contributor.authorSánchez-Quinteiro, Pablo
dc.contributor.authorRamirez, Daniel
dc.contributor.authorRodríguez, Maria Esther
dc.contributor.authorArias-Pérez, Alberto
dc.contributor.authorCross, Ismael
dc.contributor.authorDuncan, Neil
dc.contributor.authorMartínez-Peña, Teresa
dc.contributor.authorRiaza, Ana
dc.contributor.authorMillán, Adrian
dc.contributor.authorDe Rosa, M.Cristina
dc.contributor.authorPirolli, Davide
dc.contributor.authorGut, Marta
dc.contributor.authorBouza, Carmen
dc.contributor.authorRobledo, Diego
dc.contributor.authorRebordinos, Laureana
dc.contributor.authorAlioto, Tyler
dc.contributor.authorRuíz-Rejón, Carmelo
dc.contributor.authorMartínez, Paulino
dc.contributor.otherProducció Animalca
dc.date.accessioned2023-02-24T09:32:20Z
dc.date.available2023-02-24T09:32:20Z
dc.date.issued2023-01-01
dc.identifier.citationde la Herrán, Roberto, Miguel Hermida, Juan Andres Rubiolo, Jèssica Gómez-Garrido, Fernando Cruz, Francisca Robles, Rafael Navajas-Pérez, et al. "A Chromosome-Level Genome Assembly Enables the Identification of the Follicule Stimulating Hormone Receptor as the Master Sex-Determining Gene in the Flatfish Solea Senegalensis." Molecular Ecology Resources . doi:10.1111/1755-0998.13750ca
dc.identifier.issn1755-098Xca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/2123
dc.description.abstractSex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 SNP variants in the follicle stimulating hormone receptor (fshr) consistent with an XX / XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. Fshr displayed differential gene expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 non-synonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.ca
dc.format.extent19ca
dc.language.isoengca
dc.publisherWileyca
dc.relation.ispartofMolecular Ecology Reseourcesca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleA chromosome-level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex determining gene in the flatfish Solea senegalensisca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMICIU/Programa Estatal de I+D+i orientada a los retos de la sociedad/RTI2018-096847-B-C21/ES/Estudios genómicos y de determinación sexual en el lenguado (Solea senegalensis) mediante integración de mapas/ca
dc.relation.projectIDMICIU/Programa Estatal de I+D+i orientada a los retos de la sociedad/RTI2018-096847-B-C22/ES/Desarrollo del mapa de ligamento diploide del lenguado y caracterización de secuencias repetidas y genes con expresión diferencial entre sexos/ca
dc.relation.projectIDMICIU/Programa Estatal de I+D+i orientada a los retos de la sociedad/RTI2018-097110-B-C21/ES/Análisis de crecimiento y anomalías vertebrales en lenguado senegalés (Solea senegalensis): búsqueda de biomarcadores de calidad y bienestar en piscicultura/ca
dc.relation.projectIDEC/H2020/817923/EU/Advancing European Aquaculture by Genome Functional Annotation/AQUA-FAANGca
dc.subject.udc637ca
dc.identifier.doihttps://doi.org/10.1111/1755-0998.13750ca
dc.contributor.groupAqüiculturaca


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