Influence of ultrasonic application on the enzymatic formation of zinc protoporphyrin
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Publication date
2023-02-03ISSN
0260-8774
Abstract
Ferrochelatase (FeCH), present in pork liver, catalyses the formation of zinc-protoporphyrin (ZnPP), a stable purple-red pigment found in Parma ham. Kinetics of ZnPP formation is especially slow. Thus, the aim of this study was to improve the ZnPP production using power ultrasound (US) at low (7.05 W/L) and moderate power (36.53 W/L) in homogenised pork liver (HLi) and with added oxyhemoglobin (HLi + OxyHb). ZnPP formation was performed at 37 °C, under anaerobic conditions, for different reaction times (6–48 h). When US was applied at low power, it shortened the time needed to reach maximum ZnPP formation by 50% and increased the yield by 25.77% in the case of HLi and by 4.42% in that of HLi + OxyHb, when compared to the control experiments; however, for the moderate power, ZnPP formation was hindered. Therefore, low power US proved to be an effective method with which to intensify enzymatic ZnPP production.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
663/664 - Food and nutrition. Enology. Oils. Fat
Pages
7
Publisher
Elsevier
Is part of
Journal of Food Engineering
Citation
Abril, B., M. Contreras, R. Bou, M. Llauger, J.V. García-Pérez, and J. Benedito. 2023. "Influence Of Ultrasonic Application On The Enzymatic Formation Of Zinc Protoporphyrin". Journal Of Food Engineering 347: 111449. doi:10.1016/j.jfoodeng.2023.111449.
Grant agreement number
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2017-00024-C04-03/ES/Tecnologías emergentes para la obtención de zinc-protoporfirina y proteinas funcionales a partir de co-productos cárnicos/
Program
Funcionalitat i Seguretat Alimentària
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2685]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/