Separation of α-Lactalbumin Enriched Fraction from Bovine Native Whey Concentrate by Combining Membrane and High-Pressure Processing
Ver/Abrir
Fecha de publicación
2023-01-19ISSN
2304-8158
Resumen
Whey exhibits interesting nutritional properties, but its high β-Lactoglobulin (β-Lg) content could be a concern in infant food applications. In this study, high-pressure processing (HPP) was assessed as a β-Lg removal strategy to generate an enriched α-Lactalbumin (α-La) fraction from bovine native whey concentrate. Different HPP treatment parameters were considered: initial pH (physiological and acidified), sample temperature (7–35 °C), pressure (0–600 MPa) and processing time (0–490 s). The conditions providing the best α-La yield and α-La purification degree balance (46.16% and 80.21%, respectively) were 4 min (600 MPa, 23 °C), despite the significant decrease of the surface hydrophobicity and the total thiol content indexes in the α-La-enriched fraction. Under our working conditions, the general effects of HPP on α-La and β-Lg agreed with results reported in other studies of cow milk or whey. Notwithstanding, our results also indicated that the use of native whey concentrate could improve the β-Lg precipitation degree and the α-La purification degree, in comparison to raw milk or whey. Future studies should include further characterization of the α-La-enriched fraction and the implementation of membrane concentration and HPP treatment to valorize cheese whey.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
663/664 - Alimentos y nutrición. Enología. Aceites. Grasas
Páginas
17
Publicado por
MDPI
Publicado en
Foods
Citación
Romo, María, Massimo Castellari, Dinar Fartdinov, and Xavier Felipe. 2023. "Separation Of Α-Lactalbumin Enriched Fraction From Bovine Native Whey Concentrate By Combining Membrane And High-Pressure Processing". Foods 12 (3): 480. doi:10.3390/foods12030480.
Número del acuerdo de la subvención
EC/H2020/1833/EU/Dairy Innovation for Mediterranean SME/DAINME-SME
Program
Funcionalitat i Seguretat Alimentària
Qualitat i Tecnologia Alimentària
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