Male germ cell proliferation and apoptosis in sexually immature meagre Argyrosomus regius (Asso, 1801) treated with recombinant follicle stimulating hormone
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Author
Zupa, Rosa
Giménez, Ignacio
Mylonas, Constantinos C.
Pousis, Chrysovalentinos
Passantino, Letizia
Cuko, Rezart
Corriero, Aldo
Publication date
2023-04-28ISSN
2045-2322
Abstract
The meagre Argyrosomus regius (Asso, 1801) is a marine fish species that has an increasing aquaculture production in Europe. Lowering the age at maturity of hatchery-produced juveniles would support meagre aquaculture by reducing time between generations in selective breeding programs and reducing industrial costs for broodstock maintenance. The aim of this work was to assess the effects of a treatment with recombinant follicle stimulating hormone (rFsh), produced in ovarian cells of Chinese hamsters, on male germ cell proliferation and apoptosis in sexually immature meagre. The rFsh-treated fish had higher gonadosomatic index, larger seminiferous tubules, more abundant luminal spermatozoa, a lower density of anti-PCNA positive single A spermatogonia, a higher density of anti-PCNA positive spermatocysts and a lower incidence of germ cell apoptosis than control groups. The present study demonstrated the effectiveness of the produced rFsh in stimulating testis development and spermatogenesis in pre-pubertal meagre. Moreover, the rFsh treatment proved to be highly efficient in removing the apoptotic block of spermatogenesis observed in juvenile meagre, allowing spermatogonial survival and progress towards meiosis. The administration of rFsh did not stimulate spermatogonial self-renewal, a process whose control still needs to be elucidated.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
637 - Produce of domestic (farmyard) animals and game
Pages
13
Publisher
Springer Nature
Is part of
Scientific Reports
Citation
Zupa, Rosa, Neil Duncan, Ignacio Giménez, Constantinos C. Mylonas, Chrysovalentinos Pousis, Letizia Passantino, Rezart Cuko, and Aldo Corriero. 2023. "Male Germ Cell Proliferation And Apoptosis In Sexually Immature Meagre Argyrosomus Regius (Asso, 1801) Treated With Recombinant Follicle Stimulating Hormone". Scientific Reports 13 (1). doi:10.1038/s41598-023-34102-1.
Grant agreement number
EC/H2020/862658/EU/New Technologies, Tools and Strategies for a Sustainable, Resilient and Innovative European Aquaculture/NewTechAqua
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/