Revisiting the taxonomical classification of Porcine Circovirus type 2 (PCV2): still a real challenge
Visualitza/Obre
Autor/a
Franzo, Giovanni
Cortey, Martí
Olvera, Alex
Novosel, Dinko
De Castro, Alessandra Marnie Martins Gomes
Biagini, Philippe
Drigo, Michele
Data de publicació
2015-08-28ISSN
1743-422X
Resum
Background: PCV2 has emerged as one of the most devastating viral infections of swine farming, causing a relevant economic impact due to direct losses and control strategies expenses. Epidemiological and experimental studies have evidenced that genetic diversity is potentially affecting the virulence of PVC2. The growing number of PCV2 complete genomes and partial sequences available at GenBank questioned the accepted PCV2 classification.Methods: Nine hundred seventy five PCV2 complete genomes and 1,270 ORF2 sequences available from GenBank were subjected to recombination, PASC and phylogenetic analyses and results were used for comparison with previous classification scheme.
Results: The outcome of these analyses favors the recognition of four genotypes on the basis of ORF2 sequences, namely PCV2a, PCV2b, PCV2c and PCV2d-mPCV2b. To deal with the difficulty of founding an unambiguous classification and accounting the impossibility to define a p-distance cut-off, a set of reference sequences that could be used in further phylogenetic studies for PCV2 genotyping was established. Being aware that extensive phylogenetic analyses are time-consuming and often impracticable during routine diagnostic activity, ORF2 nucleotide positions adequately conserved in the reference sequences were identified and reported to allow a quick genotype differentiation. Conclusions: Globally, the present work provides an updated scenario of PCV2 genotypes distribution and, based
on the limits of the previous classification criteria, proposes new rapid and effective schemes for differentiating the four defined PCV2 genotypes.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
8
Publicat per
BMC
Publicat a
Virology Journal
Citació
Franzo, Giovanni, Martí Cortey, Alex Olvera, Dinko Novosel, Alessandra Marnie Martins Gomes De Castro, Philippe Biagini, Joaquim Segalés, and Michele Drigo. 2015. "Revisiting The Taxonomical Classification Of Porcine Circovirus Type 2 (PCV2): Still A Real Challenge". Virology Journal 12 (1). doi:10.1186/s12985-015-0361-x.
Programa
Sanitat Animal
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