Postnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implications
Visualitza/Obre
Autor/a
Muñoz-González, Sara
Ruggli, Nicolas
Pérez, Lester Josué
Frías-Leuporeau, Maria Teresa
Fraile, Lorenzo
Cordoba, Lorena
Domingo, Mariano
Ehrensperger, Felix
Summerfield, Artur
Data de publicació
2015-05-04ISSN
1932-6203
Resum
It is well established that trans-placental transmission of classical swine fever virus (CSFV) during mid-gestation can lead to persistently infected offspring. The aim of the present study was to evaluate the ability of CSFV to induce viral persistence upon early postnatal infection. Two litters of 10 piglets each were infected intranasally on the day of birth with low and moderate virulence CSFV isolates, espectively. During six weeks after postnatal infection, most of the piglets remained clinically healthy, despite persistent high virus titres in the serum. Importantly, these animals were unable to mount any detectable humoral and cellular immune response. At necropsy, the most prominent gross pathological lesion was a severe thymus atrophy. Four weeks after infection, PBMCs from the persistently infected seronegative piglets were unresponsive to both, specific CSFV and non-specific PHA stimulation in terms of IFN-γ-producing cells. These results suggested the development of a state of immunosuppression in these postnatally persistently infected pigs. However, IL-10 was undetectable in the sera of the persistently infected animals. Interestingly, CSFV-stimulated PBMCs from the persistently infected piglets produced IL-10. Nevertheless, despite the addition of the anti-IL-10 antibody in the PBMC culture from persistently infected piglets, the response of the IFN-γ producing cells was not restored. Therefore, other factors than IL-10 may be involved in the general suppression of the T-cell responses upon CSFV and mitogen activation. Interestingly, bone marrow immature granulocytes were increased and targeted by the virus in persistently infected piglets. Taken together, we provided the first data demonstrating the feasibility of CSFV in generating a postnatal persistent disease, which has not been shown for other members of the Pestivirus genus yet. Since serological methods are routinely used in CSFV surveillance, persistently infected pigs might go unnoticed. In addition to the epidemiological and economic significance of persistent CSFV infection, this model could be useful for understanding the mechanisms of viral persistence.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
22
Publicat per
Public Library of Science
Publicat a
PLoS ONE
Citació
Muñoz-González, S., Ruggli, N., Rosell, R., Pérez, L. J., Frías-Leuporeau, M. T., Fraile, L., Montoya, M., Cordoba, L., Domingo, M., Ehrensperger, F., Summerfield, A. and Ganges, L. Muñoz-González, Sara, Nicolas Ruggli, Rosa Rosell, Lester Josué Pérez, Maria Teresa Frías-Leuporeau, Lorenzo Fraile, and Maria Montoya et al. 2015. "Postnatal Persistent Infection With Classical Swine Fever Virus And Its Immunological Implications". PLOS ONE 10 (5): e0125692. doi:10.1371/journal.pone.0125692.
Número de l'acord de la subvenció
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-38343/ES/ESTUDIOS DE INMUNOPATOGENICIDAD FRENTE AL VIRUS DE LA PESTE PORCINA CLASICA (VPPC): IMPLICACIONES PARA EL DESARROLLO DE NUEVAS VACUNAS Y HERRAMIENTAS DIAGNOSTICAS/
Programa
Sanitat Animal
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