The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
Visualitza/Obre
Autor/a
Zapater, Cinta
Stavang, Jon Anders
Taranger, Geir Lasse
Finn, Roderick Nigel
Data de publicació
2015-02-09ISSN
0892-6638
Resum
Water homeostasis and the structural integrity of the vertebrate lens is partially mediated by AQP0
channels. Emerging evidence indicates that external pH may be involved in channel gating. Here we show that a tetraploid teleost, the Atlantic salmon, retains 4 aqp0 genes (aqp0a1, -0a2, -0b1, and -0b2), which are highly, but not exclusively, expressed in the lens. Functional characterization reveals that,although each paralog permeateswater efficiently, the permeability is respectively shifted to the
neutral, alkaline, or acidic pH in Aqp0a1, -0a2, and -0b1, whereas that of Aqp0b2 is not regulated by external pH. Mutagenesis studies demonstrate that Ser38, His39, and His40 residues in the extracellular transmembrane domain of a-helix 2 facing the water pore are critical for the pH modulation of water transport. To validate these findings, we show that both zebrafish Aqp0a and -0b are functional water channels with respective pH sensitivities toward alkaline or acid pH ranges and that an N-terminal allelic
variant (Ser19) of Aqp0b exists that abolishes water transport in Xenopus laevis oocytes. The data suggest that the alkaline pH sensitivity is a conserved trait in teleost Aqp0 a-type channels, whereas mammalian AQP0 and some teleost Aqp0 b-type channels display an acidic pH permeation preference.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
639 - Caça. Pesca. Piscicultura
Pàgines
13
Publicat per
Wiley Open Access
Publicat a
FASEB Journal
Citació
Chauvigne, François, Cinta Zapater, Jon Anders Stavang, Geir Lasse Taranger, Joan Cerda, and Roderick Nigel Finn. 2015. “The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts”. FASEB Journal 29(5). doi:10.1096/fj.14-267625
Número de l'acord de la subvenció
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-15597/ES/AQUAPORINAS TESTICULARES DURANTE LA ESPERMATOGENESIS DE PECES MARINOS Y LA ACTIVACION Y PERMEABILIDAD ESPERMATICA/
Programa
Aqüicultura
Aquest element apareix en la col·lecció o col·leccions següent(s)
- ARTICLES CIENTÍFICS [2650]
Excepte que s'indiqui una altra cosa, la llicència de l'ítem es descriu com http://creativecommons.org/licenses/by-nc/4.0/