Gestational Caloric Restriction Alters Adipose Tissue Methylome and Offspring’s Metabolic Profile in a Swine Model
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Author
Mas-Parés, Berta
Xargay-Torrent, Sílvia
Carreras-Badosa, Gemma
Gómez-Vilarrubla, Ariadna
Niubó-Pallàs, Maria
Reixach, Josep
Prats-Puig, Anna
de Zegher, Francis
Ibañez, Lourdes
Bassols, Judit
López-Bermejo, Abel
Publication date
2024-01-17ISSN
1661-6596
Abstract
Limited nutrient supply to the fetus results in physiologic and metabolic adaptations that have unfavorable consequences in the offspring. In a swine animal model, we aimed to study the effects of gestational caloric restriction and early postnatal metformin administration on offspring’s adipose tissue epigenetics and their association with morphometric and metabolic variables. Sows were either underfed (30% restriction of total food) or kept under standard diet during gestation, and piglets were randomly assigned at birth to receive metformin (n = 16 per group) or vehicle treatment (n = 16 per group) throughout lactation. DNA methylation and gene expression were assessed in the retroperitoneal adipose tissue of piglets at weaning. Results showed that gestational caloric restriction had a negative effect on the metabolic profile of the piglets, increased the expression of inflammatory markers in the adipose tissue, and changed the methylation of several genes related to metabolism. Metformin treatment resulted in positive changes in the adipocyte morphology and regulated the methylation of several genes related to atherosclerosis, insulin, and fatty acids signaling pathways. The methylation and gene expression of the differentially methylated FASN, SLC5A10, COL5A1, and PRKCZ genes in adipose tissue associated with the metabolic profile in the piglets born to underfed sows. In conclusion, our swine model showed that caloric restriction during pregnancy was associated with impaired inflammatory and DNA methylation markers in the offspring’s adipose tissue that could predispose the offspring to later metabolic abnormalities. Early metformin administration could modulate the size of adipocytes and the DNA methylation changes.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
636 - Animal husbandry and breeding in general. Livestock rearing. Breeding of domestic animals
Pages
15
Publisher
MDPI
Is part of
International Journal of Molecular Sciences
Citation
Mas-Parés, Berta, Sílvia Xargay-Torrent, Gemma Carreras‐Badosa, Ariadna Gómez-Vilarrubla, Maria Niubó-Pallàs, Joan Tibau and Josep Reixach et al. 2024. “Gestational caloric restriction alters adipose tissue methylome and offspring’s metabolic profile in a swine model”. International Journal of Molecular Sciences, 25(2): 1128. doi:10.3390/ijms25021128.
Grant agreement number
ISCIII/ /PI20-00399/ES/Análisis longitudinal de las marcas de metilación del DNA asociadas con la obesidad infantil/
ISCIII/ /PI19-00451/ES/La impronta genética en el crecimiento postnatal. Impacto de la obesidad parental/
ISCIII/ /PI22-00366/ES/Epigenética en niñas con pubertad adelantada y predicción de su evolución puberal/
FEDER/ / /EU/ /
Program
Genètica i Millora Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/