High-Fat Diet-Induced Obesity Increases Brain Mitochondrial Complex I and Lipoxidation-Derived Protein Damage
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Autor/a
Berdún, Rebeca
Obis, Èlia
Mota-Martorell, Natàlia
Bassols, Anna
Valent, Daniel
Serrano, José C. E.
Martín-Garí, Meritxell
Rodríguez-Palmero, María
Moreno-Muñoz, José Antonio
Pamplona, Reinald
Portero-Otín, Manuel
Jové, Mariona
Fecha de publicación
2024-01-26ISSN
2076-3921
Resumen
Obesity is a risk factor for highly prevalent age-related neurodegenerative diseases, the pathogenesis of whichinvolves mitochondrial dysfunction and protein oxidative damage. Lipoxidation, driven by high levels of peroxidizable unsaturated fatty acids and low antioxidant protection of the brain, stands out as a significant risk factor. To gain information on the relationship between obesity and brain molecular damage, in a porcine model of obesity we evaluated (1) the level of mitochondrial respiratory chain complexes, as the main source of free radical generation, by Western blot; (2) the fatty acid profile by gas chromatography; and (3) the oxidative modification of proteins by mass spectrometry. The results demonstrate a selectively higher amount of the lipoxidation-derived biomarker malondialdehyde-lysine (MDAL) (34% increase) in the frontal cortex, and positive correlations between MDAL and LDL levels and body weight. No changes were observed in brain fatty acid profile by the high-fat diet, and the increased lipid peroxidative modification was associated with increased levels of mitochondrial complex I (NDUFS3 and NDUFA9 subunits) and complex II (flavoprotein). Interestingly, introducing n3 fatty acids and a probiotic in the high-fat diet prevented the observed changes, suggesting that dietary components can modulate protein oxidative modification at the cerebral level and opening new possibilities in neurodegenerative diseases’ prevention.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
576 - Biología celular y subcelular. Citología
Páginas
15
Publicado por
MDPI
Publicado en
Antioxidants
Citación
Berdún, Rebeca, Èlia Obis, Natàlia Mota-Martorell, Anna Bassols, Daniel Valent, José C. E. Serrano, Meritxell Martín-Garí, et al. 2024. “High-Fat Diet-Induced Obesity Increases Brain Mitochondrial Complex I and Lipoxidation-Derived Protein Damage.” Antioxidants 13(2):161. doi:10.3390/antiox13020161.
Número del acuerdo de la subvención
CDTI/ /IDI-20141216/ES/ /SMARTFOODS
FEDER/ / /EU/ /
CDTI/ /IPT-20111008/ES/Guía para la sustanciación de declaraciones de salud en alimentos: funciones inmune, cognitiva y síndrome metabólico/INCOMES
ISCIII/ /PI20-000155/ES/ /
Program
Genètica i Millora Animal
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