Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells
Author
Torrealba, Débora
López, Daniela
Zelada, Patricio
Salinas-Parra, Nicolás
Valenzuela-Avilés, Paula
Mercado, Luis
Altamirano, Claudia
Gallardo-Matus, José
Publication date
2024-03-09ISSN
2076-2615
Abstract
Recent studies have demonstrated that immune-related recombinant proteins can enhance immune function, increasing host survival against infectious diseases in salmonids. This research evaluated inclusion bodies (IBs) of antimicrobial peptides (CAMPIB and HAMPIB) and a cytokine (IL1βIB and TNFαIB) as potential immunostimulants in farmed salmonids. For this purpose, we produced five IBs (including iRFPIB as a control), and we evaluated their ability to modulate immune marker gene expression of three IBs in the RTS11 cell line by RT–qPCR. Additionally, we characterized the scale-up of IBs production by comparing two different scale systems. The results showed that CAMPIB can increase the upregulation of tnfα, il1β, il8, and il10, HAMPIB significantly increases the upregulation of tnfα, inos, and il10, and IL1βIB significantly upregulated the expression of tnfα, il1β, and cox2. A comparison of IL1βIB production showed that the yield was greater in shake flasks than in bioreactors (39 ± 1.15 mg/L and 14.5 ± 4.08 mg/L), and larger nanoparticles were produced in shake flasks (540 ± 129 nm and 427 ± 134 nm, p < 0.0001, respectively). However, compared with its shake flask counterpart, the IL1βIB produced in a bioreactor has an increased immunomodulatory ability. Further studies are needed to understand the immune response pathways activated by IBs and the optimal production conditions in bioreactors, such as a defined medium, fed-batch production, and mechanical bacterial lysis, to increase yield.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
639 - Hunting. Fishing. Fish breeding
Pages
20
Publisher
MDPI
Is part of
Animals
Citation
Torrealba, Débora, Daniela López, Patricio Zelada, Nicolás Salinas-Parra, Paula Valenzuela-Avilés, Elena Garcia-Fruitós, Anna Arís, Luis Mercado, Claudia Altamirano, and José Gallardo-Matus. 2024. “Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells.” Animals 14. doi:10.3390/ani14060844.
Program
Producció de Remugants
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/