Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells
Autor/a
Torrealba, Débora
López, Daniela
Zelada, Patricio
Salinas-Parra, Nicolás
Valenzuela-Avilés, Paula
Mercado, Luis
Altamirano, Claudia
Gallardo-Matus, José
Fecha de publicación
2024-03-09ISSN
2076-2615
Resumen
Recent studies have demonstrated that immune-related recombinant proteins can enhance immune function, increasing host survival against infectious diseases in salmonids. This research evaluated inclusion bodies (IBs) of antimicrobial peptides (CAMPIB and HAMPIB) and a cytokine (IL1βIB and TNFαIB) as potential immunostimulants in farmed salmonids. For this purpose, we produced five IBs (including iRFPIB as a control), and we evaluated their ability to modulate immune marker gene expression of three IBs in the RTS11 cell line by RT–qPCR. Additionally, we characterized the scale-up of IBs production by comparing two different scale systems. The results showed that CAMPIB can increase the upregulation of tnfα, il1β, il8, and il10, HAMPIB significantly increases the upregulation of tnfα, inos, and il10, and IL1βIB significantly upregulated the expression of tnfα, il1β, and cox2. A comparison of IL1βIB production showed that the yield was greater in shake flasks than in bioreactors (39 ± 1.15 mg/L and 14.5 ± 4.08 mg/L), and larger nanoparticles were produced in shake flasks (540 ± 129 nm and 427 ± 134 nm, p < 0.0001, respectively). However, compared with its shake flask counterpart, the IL1βIB produced in a bioreactor has an increased immunomodulatory ability. Further studies are needed to understand the immune response pathways activated by IBs and the optimal production conditions in bioreactors, such as a defined medium, fed-batch production, and mechanical bacterial lysis, to increase yield.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
639 - Caza. Pesca. Piscicultura
Páginas
20
Publicado por
MDPI
Publicado en
Animals
Citación
Torrealba, Débora, Daniela López, Patricio Zelada, Nicolás Salinas-Parra, Paula Valenzuela-Avilés, Elena Garcia-Fruitós, Anna Arís, Luis Mercado, Claudia Altamirano, and José Gallardo-Matus. 2024. “Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells.” Animals 14. doi:10.3390/ani14060844.
Program
Producció de Remugants
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