dc.contributor.author | Mohamed, Walid | |
dc.contributor.author | Sethi, Shneh | |
dc.contributor.author | Tchatalbachev, Svetlin | |
dc.contributor.author | Darji, Ayub | |
dc.contributor.author | Chakraborty, Trinad | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2024-04-04T12:58:54Z | |
dc.date.available | 2024-04-04T12:58:54Z | |
dc.date.issued | 2012-04-19 | |
dc.identifier.citation | Mohamed, Walid, Shneh Sethi, Svetlin Tchatalbachev, Ayub Darji, and Trinad Chakraborty. 2012. “Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria Innocua Harboring the VGC Locus.” PloS One 7 (4): e35503. doi:10.1371/journal.pone.0035503. | ca |
dc.identifier.issn | 1932-6203 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/2902 | |
dc.description.abstract | In this study we propose a novel bacterial vaccine strategy where non-pathogenic bacteria are complemented with traits
desirable for the induction of protective immunity. To illustrate the proof of principle of this novel vaccination strategy, we
use the model organism of intracellular immunity Listeria. We introduced a, low copy number BAC-plasmid harbouring the
virulence gene cluster (vgc) of L. monocytogenes (Lm) into the non-pathogenic L. innocua (L.inn) strain and examined for its
ability to induce protective cellular immunity. The resulting strain (L.inn::vgc) was attenuated for virulence in vivo and
showed a strongly reduced host detrimental inflammatory response compared to Lm. Like Lm, L.inn::vgc induced the
production of Type I Interferon’s and protection was mediated by Listeria-specific CD8+ T cells. Rational vaccine design
whereby avirulent strains are equipped with the capabilities to induce protection but lack detrimental inflammatory effects
offer great promise towards future studies using non-pathogenic bacteria as vectors for vaccination. | ca |
dc.description.sponsorship | The work was funded by the Federal Ministry of Education and Research (BMBF) through the ERA-NET Pathogenomics SPATELIS project. This funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ca |
dc.format.extent | 9 | ca |
dc.language.iso | eng | ca |
dc.publisher | Public Library of Science | ca |
dc.relation.ispartof | PLoS ONE | ca |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0035503 | ca |
dc.contributor.group | Sanitat Animal | ca |