Segregation of Regulatory Polymorphisms with Effects on the Gluteus Medius Transcriptome in a Purebred Pig Population
Ver/Abrir
Autor/a
Cánovas, Angela
Gallardo, David
Ramırez, Oscar
Amills, Marcel
Fecha de publicación
2012-04-24ISSN
1932-6203
Resumen
Background: The main goal of the present study was to analyse the genetic architecture of mRNA expression in muscle, a
tissue with an outmost economic importance for pig breeders. Previous studies have used F2 crosses to detect porcine
expression QTL (eQTL), so they contributed with data that mostly represents the between-breed component of eQTL
variation. Herewith, we have analysed eQTL segregation in an outbred Duroc population using two groups of animals with
divergent fatness profiles. This approach is particularly suitable to analyse the within-breed component of eQTL variation,
with a special emphasis on loci involved in lipid metabolism.
Methodology/Principal Findings: GeneChip Porcine Genome arrays (Affymetrix) were used to determine the mRNA
expression levels of gluteus medius samples from 105 Duroc barrows. A whole-genome eQTL scan was carried out with a
panel of 116 microsatellites. Results allowed us to detect 613 genome-wide significant eQTL unevenly distributed across the
pig genome. A clear predominance of trans- over cis-eQTL, was observed. Moreover, 11 trans-regulatory hotspots affecting
the expression levels of four to 16 genes were identified. A Gene Ontology study showed that regulatory polymorphisms
affected the expression of muscle development and lipid metabolism genes. A number of positional concordances between
eQTL and lipid trait QTL were also found, whereas limited evidence of a linear relationship between muscle fat deposition
and mRNA levels of eQTL regulated genes was obtained.
Conclusions/Significance: Our data provide substantial evidence that there is a remarkable amount of within-breed genetic
variation affecting muscle mRNA expression. Most of this variation acts in trans and influences biological processes related
with muscle development, lipid deposition and energy balance. The identification of the underlying causal mutations and
the ascertainment of their effects on phenotypes would allow gaining a fundamental perspective about how complex traits
are built at the molecular level.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
575 - Genética general. Citogenética general. Inmunogenética. Evolución. Filogenia
636 - Explotación y cría de animales. Cría del ganado y de animales domésticos
Páginas
12
Publicado por
Public Library of Science
Publicado en
PLoS ONE
Citación
Cánovas, Ángela, Ramona N. Pena, David Gallardo, Óscar Ramírez, Marcel Amills, and Raquel Quintanilla. 2012. “Segregation of Regulatory Polymorphisms With Effects on the Gluteus Medius Transcriptome in a Purebred Pig Population.” PLOS ONE 7 (4): e35583. doi:10.1371/journal.pone.0035583.
Número del acuerdo de la subvención
MICYT//GEN2003-20658-C05-05/ES/Análisis genómico mediante microarrays de la arquitectura genética de fenotipos complejos en porcino/
MEC/ /AGL2007-66707-C02-01/ES/Mapeo e identificación de genes implicados en el metabolismo lipídico en porcino, la calidad de la carne y la calidad del jamón curado/
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22208-C02-01/ES/Utilización de aceites ácidos esterificados en la alimentación de animales monogástricos. Nutrición comparada y repercusiones sobre la calidad lipídica de la carne/ESTERIFATS
Program
Genètica i Millora Animal
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2685]
Excepto si se señala otra cosa, la licencia del ítem se describe como http://creativecommons.org/licenses/by/4.0/