β-cyclodextrin polymer as tetrodotoxins scavenger in oyster extracts
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Author
Fragoso, Alex
Torréns, Mabel
Klijnstra, Mirjam
Gerssen, Arjen
Publication date
2024-04-16ISSN
0026-265X
Abstract
Tetrodotoxins (TTXs) are potent marine neurotoxins involved in poisoning cases in humans after consumption of some marine organisms. A cell-based assay (CBA) providing toxicological information is a commonly used method for their detection because of its high sensitivity. However, matrix effects may interfere in the CBA. In this work, five insoluble cyclodextrin polymers, with different chemicals structures (βCDP-14-DS2, βCDP-13-DS2, βCDP-13-DS4, βCDP-14-DS4 and βCDP-N-DS5) have been investigated as novel clean-up materials for oyster extracts containing TTXs. The best recoveries have been achieved with βCDP-14-DS2 (∼100 %), which allowed exposing cells to 27-fold higher oyster matrix concentrations and decreased the LOQ down to 46 µg equiv. TTX/kg. Then, the applicability of this strategy has been demonstrated with oyster extracts from The Netherlands and comparing the results with immunoassay and liquid chromatography-tandem mass spectrometry (LC-MS/MS). This clean-up strategy combined with CBA could be implemented for TTXs detection in monitoring programs to ensure seafood safety.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
27
Publisher
Elsevier
Is part of
Microchemical Journal
Citation
Alkassar, Mounira, Jaume Reverté, Alex Fragoso, Mabel Torréns, Mirjam D. Klijnstra, Arjen Gerssen, Jorge Diogène, and Mònica Campàs. 2024. “Β-cyclodextrin Polymer as Tetrodotoxins Scavenger in Oyster Extracts.” Microchemical Journal, 110585. https://doi.org/10.1016/j.microc.2024.110585.
Grant agreement number
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
EC/HORIZON/101086234/EU/Solutions to prevent and mitigate the impacts of HABs in Aquaculture and Fisheries, in the context of global warming/BlueShellfish
Program
Aigües Marines i Continentals
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2838]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/