Multidrug resistance in Salmonella isolates of swine origin: mobile genetic elements and plasmids associated with cephalosporin resistance with potential transmission to humans
Ver/Abrir
Autor/a
Garrido, V.
Arrieta-Gisasola, A.
Migura-García, L.
Laorden, L.
Grilló, M. J.
Fecha de publicación
2024-05-02ISSN
0099-2240
Resumen
The emergence of foodborne Salmonella strains carrying antimicrobial resistance (AMR) in mobile genetic elements (MGE) is a significant public health threat in a One Health context requiring continuous surveillance. Resistance to ciprofloxacin and cephalosporins is of particular concern. Since pigs are a relevant source of foodborne Salmonella for human beings, we studied transmissible AMR genes and MGE in a collection of 83 strains showing 9 different serovars and 15 patterns of multidrug resistant (MDR) previously isolated from pigs raised in the conventional breeding system of Northern Spain. All isolates were susceptible to ciprofloxacin and three isolates carried blaCMY-2 or blaCTX-M-9 genes responsible for cefotaxime resistance. Filter mating experiments showed that the two plasmids carrying blaCTX-M-9 were conjugative while that carrying blaCMY-2 was self-transmissible by transformation. Whole-genome sequencing and comparative analyses were performed on the isolates and plasmids. The IncC plasmid pSB109, carrying blaCMY-2, was similar to one found in S. Reading from cattle, indicating potential horizontal transfer between serovars and animal sources. The IncHI2 plasmids pSH102 in S. Heidelberg and pSTM45 in S. Typhimurium ST34, carrying blaCTX-M-9, shared similar backbones and two novel “complex class 1 integrons” containing different AMR and heavy metal genes. Our findings emphasize the importance of sequencing techniques to identify emerging AMR regions in conjugative and stable plasmids from livestock production. The presence of MGE carrying clinically relevant AMR genes raises public health concerns, requiring monitoring to mitigate the emergence of bacteria carrying AMR genes and subsequent spread through animals and food.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
619 - Veterinaria
Páginas
32
Publicado por
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology
Citación
Garrido, V., A. Arrieta-Gisasola, L. Migura-García, L. Laorden, and M. J. Grilló. 2024. “Multidrug Resistance in Salmonella Isolates of Swine Origin: Mobile Genetic Elements and Plasmids Associated With Cephalosporin Resistance With Potential Transmission to Humans.” Applied and Environmental Microbiology 90(5):e00264-24 https://doi.org/10.1128/aem.00264-24.
Program
Sanitat Animal
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