Virulence-associated trimeric autotransporters of Haemophilus parasuis are antigenic proteins expressed in vivo
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Data de publicació
2009-12-10ISSN
0928-4249
Resum
Glässer’s disease is a re-emerging swine disease characterized by a severe septicaemia.
Vaccination has been widely used to control the disease, although there is a lack of extended crossprotection. Trimeric autotransporters, a family of surface exposed proteins implicated in host-pathogen
interactions, are good vaccine candidates. Members of this family have been described in Haemophilus
parasuis and designated as virulence-associated trimeric autotransporters (VtaA). In this work, we produced
15 recombinant VtaA passenger domains and looked for the presence of antibodies directed against them in
immune sera by immunoblotting. After infection with a subclinical dose of H. parasuis Nagasaki, an IgG
mediated antibody response against 6 (VtaA1, 5, 6, 8, 9 and 10) of the 13 VtaA of the Nagasaki strain was
detected, indicating that they are expressed in vivo. IgA production against VtaA was detected in only one
animal. VtaA were more likely to be late antigens when compared to early (Omp P5 and Omp P6) and late
(YaeT) defined antigens. Antibody cross-reaction with two orthologs of Nagasaki’s VtaA5 and 6, VtaA15
and 16 of strain HP1319, was also detected. No antibodies against VtaA were detected in the sera of animals
immunized with a bacterin of the Nagasaki strain, suggesting poor expression in the in vitro conditions
used. Taken together, these results indicate that VtaA are good candidate immunogens that could be used to
improve H. parasuis vaccines. However, their capacity to confer protective immunity needs to be further
studied.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
11
Publicat per
EDP Sciences
Publicat a
Veterinary Research
Citació
Olvera, Alex, Sonia Pina, Marta Pérez-Simó, Simone Oliveira, and Albert Bensaid. 2009. “Virulence-associated Trimeric Autotransporters ofHaemophilus Parasuisare Antigenic Proteins Expressed in Vivo.” Veterinary Research 41 (3): 26. doi: 10.1051/vetres/2009074
Número de l'acord de la subvenció
MEC/ /AGL2007-60432/ES/Caracterizacion funcional e inmunologica de las proteinas de superficie y proteinas secretadas de cepas virulentas de Haemophilus parasuis/GAN
Programa
Sanitat Animal
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