Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
Visualitza/Obre
Data de publicació
2010-06-11ISSN
1471-2164
Resum
Background: Selection for increasing intramuscular fat content would definitively improve the palatability and
juiciness of pig meat as well as the sensorial and organoleptic properties of cured products. However, evidences
obtained in human and model organisms suggest that high levels of intramuscular fat might alter muscle lipid and
carbohydrate metabolism. We have analysed this issue by determining the transcriptomic profiles of Duroc pigs with
divergent phenotypes for 13 fatness traits. The strong aptitude of Duroc pigs to have high levels of intramuscular fat
makes them a valuable model to analyse the mechanisms that regulate muscle lipid metabolism, an issue with evident
implications in the elucidation of the genetic basis of human metabolic diseases such as obesity and insulin resistance.
Results: Muscle gene expression profiles of 68 Duroc pigs belonging to two groups (HIGH and LOW) with extreme
phenotypes for lipid deposition and composition traits have been analysed. Microarray and quantitative PCR analysis
showed that genes related to fatty acid uptake, lipogenesis and triacylglycerol synthesis were upregulated in the
muscle tissue of HIGH pigs, which are fatter and have higher amounts of intramuscular fat than their LOW counterparts.
Paradoxically, lipolytic genes also showed increased mRNA levels in the HIGH group suggesting the existence of a cycle
where triacylglycerols are continuously synthesized and degraded. Several genes related to the insulin-signalling
pathway, that is usually impaired in obese humans, were also upregulated. Finally, genes related to antigen-processing
and presentation were downregulated in the HIGH group.
Conclusion: Our data suggest that selection for increasing intramuscular fat content in pigs would lead to a shift but
not a disruption of the metabolic homeostasis of muscle cells. Future studies on the post-translational changes
affecting protein activity or expression as well as information about protein location within the cell would be needed
to to elucidate the effects of lipid deposition on muscle metabolism in pigs.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
575 - Genètica general. Citogenètica general. Immunogenètica. Evolució. Filogènia
Pàgines
15
Publicat per
BMC
Publicat a
BMC Genomics
Citació
Cánovas, Angela, Raquel Quintanilla, Marcel Amills, and Ramona N Pena. 2010. “Muscle Transcriptomic Profiles in Pigs With Divergent Phenotypes for Fatness Traits.” BMC Genomics 11 (1): 372. doi: 10.1186/1471-2164-11-372
Número de l'acord de la subvenció
MICYT//GEN2003-20658-C05-05/ES/Análisis genómico mediante microarrays de la arquitectura genética de fenotipos complejos en porcino/
MEC/ /AGL2007-66707-C02-01/ES/Mapeo e identificación de genes implicados en el metabolismo lipídico en porcino, la calidad de la carne y la calidad del jamón curado/
Programa
Genètica i Millora Animal
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