Mostrar el registro sencillo del ítem
Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
dc.contributor.author | Cánovas, Angela | |
dc.contributor.author | Quintanilla, Raquel | |
dc.contributor.author | Amills, Marcel | |
dc.contributor.author | Pena, Romi | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2024-08-02T13:05:31Z | |
dc.date.available | 2024-08-02T13:05:31Z | |
dc.date.issued | 2010-06-11 | |
dc.identifier.citation | Cánovas, Angela, Raquel Quintanilla, Marcel Amills, and Ramona N Pena. 2010. “Muscle Transcriptomic Profiles in Pigs With Divergent Phenotypes for Fatness Traits.” BMC Genomics 11 (1): 372. doi: 10.1186/1471-2164-11-372 | ca |
dc.identifier.issn | 1471-2164 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/3105 | |
dc.description.abstract | Background: Selection for increasing intramuscular fat content would definitively improve the palatability and juiciness of pig meat as well as the sensorial and organoleptic properties of cured products. However, evidences obtained in human and model organisms suggest that high levels of intramuscular fat might alter muscle lipid and carbohydrate metabolism. We have analysed this issue by determining the transcriptomic profiles of Duroc pigs with divergent phenotypes for 13 fatness traits. The strong aptitude of Duroc pigs to have high levels of intramuscular fat makes them a valuable model to analyse the mechanisms that regulate muscle lipid metabolism, an issue with evident implications in the elucidation of the genetic basis of human metabolic diseases such as obesity and insulin resistance. Results: Muscle gene expression profiles of 68 Duroc pigs belonging to two groups (HIGH and LOW) with extreme phenotypes for lipid deposition and composition traits have been analysed. Microarray and quantitative PCR analysis showed that genes related to fatty acid uptake, lipogenesis and triacylglycerol synthesis were upregulated in the muscle tissue of HIGH pigs, which are fatter and have higher amounts of intramuscular fat than their LOW counterparts. Paradoxically, lipolytic genes also showed increased mRNA levels in the HIGH group suggesting the existence of a cycle where triacylglycerols are continuously synthesized and degraded. Several genes related to the insulin-signalling pathway, that is usually impaired in obese humans, were also upregulated. Finally, genes related to antigen-processing and presentation were downregulated in the HIGH group. Conclusion: Our data suggest that selection for increasing intramuscular fat content in pigs would lead to a shift but not a disruption of the metabolic homeostasis of muscle cells. Future studies on the post-translational changes affecting protein activity or expression as well as information about protein location within the cell would be needed to to elucidate the effects of lipid deposition on muscle metabolism in pigs. | ca |
dc.description.sponsorship | We are thankful to Selección Batallé S.A. for providing the animal material, to J Reixach, JL Noguera and D Almuzara for their cooperation in the experimental protocol, and to I Díaz (IRTA, Tecnologia dels Aliments), JM Prat-Cuffi (Hospital de Palamós) and the Facilities in the Vall d'Hebron Research Centre for their technical support. A Cánovas received a predoctoral scholarship from INIA. RN Pena received a contractual grant from INIA. This project was financed by the Spanish Ministry of Education and Science (projects GEN2003-20658-C05-05 and AGL2007-66707-C02-01). | ca |
dc.format.extent | 15 | ca |
dc.language.iso | eng | ca |
dc.publisher | BMC | ca |
dc.relation.ispartof | BMC Genomics | ca |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MICYT//GEN2003-20658-C05-05/ES/Análisis genómico mediante microarrays de la arquitectura genética de fenotipos complejos en porcino/ | ca |
dc.relation.projectID | MEC/ /AGL2007-66707-C02-01/ES/Mapeo e identificación de genes implicados en el metabolismo lipídico en porcino, la calidad de la carne y la calidad del jamón curado/ | ca |
dc.subject.udc | 575 | ca |
dc.identifier.doi | https://doi.org/10.1186/1471-2164-11-372 | ca |
dc.contributor.group | Genètica i Millora Animal | ca |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
ARTICLES CIENTÍFICS [2.669]