Construction of an almond linkage map in an Australian population Nonpareil × Lauranne
Author
Tavassolian, Iraj
Rabiei, Gholmereza
Gregory, Davina
Wirthensohn, Michelle G
Hunt, Peter W
Gibson, John P
Ford, Christopher M
Sedgley, Margaret
Wu, Shu-Biao
Publication date
2010-10-09ISSN
1471-2164
Abstract
Background: Despite a high genetic similarity to peach, almonds (Prunus dulcis) have a fleshless fruit and edible
kernel, produced as a crop for human consumption. While the release of peach genome v1.0 provides an excellent
opportunity for almond genetic and genomic studies, well-assessed segregating populations and the respective
saturated genetic linkage maps lay the foundation for such studies to be completed in almond.
Results: Using an almond intraspecific cross between ‘Nonpareil’ and ‘Lauranne’ (N × L), we constructed a
moderately saturated map with SSRs, SNPs, ISSRs and RAPDs. The N × L map covered 591.4 cM of the genome
with 157 loci. The average marker distance of the map was 4.0 cM. The map displayed high synteny and
colinearity with the Prunus T × E reference map in all eight linkage groups (G1-G8). The positions of 14 mapped
gene-anchored SNPs corresponded approximately with the positions of homologous sequences in the peach
genome v1.0. Analysis of Mendelian segregation ratios showed that 17.9% of markers had significantly skewed
genotype ratios at the level of P < 0.05. Due to the large number of skewed markers in the linkage group 7, the
potential existence of deleterious gene(s) was assessed in the group. Integrated maps produced by two different
mapping methods using JoinMap® 3 were compared, and their high degree of similarity was evident despite the
positional inconsistency of a few markers.
Conclusions: We presented a moderately saturated Australian almond map, which is highly syntenic and collinear
with the Prunus reference map and peach genome V1.0. Therefore, the well-assessed almond population reported
here can be used to investigate the traits of interest under Australian growing conditions, and provides more
information on the almond genome for the international community.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
575 - General genetics. General cytogenetics
633 - Field crops and their production
Pages
10
Publisher
BMC
Is part of
BMC Genomics
Citation
Tavassolian, Iraj, Gholmereza Rabiei, Davina Gregory, Mourad Mnejja, Michelle G Wirthensohn, Peter W Hunt, John P Gibson, Christopher M Ford, Margaret Sedgley, and Shu-Biao Wu. 2010. “Construction of an Almond Linkage Map in an Australian Population Nonpareil × Lauranne.” BMC Genomics 11 (1): 551. doi: 10.1186/1471-2164-11-551
Program
Genòmica i Biotecnologia
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