Dietary fat source affects metabolism of fatty acids in pigs as evaluated by altered expression of lipogenic genes in liver and adipose tissues
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Publication date
2009-01-01ISSN
1751-7311
Abstract
Little is known about pig gene expressions related to dietary fatty acids (FAs) and most work have been conducted in rodents.
The aim of this study was to investigate how dietary fats regulate fat metabolism of pigs in different tissues. Fifty-six crossbred
gilts (62 6 5.2 kg BW) were fed one of seven dietary treatments (eight animals per treatment): a semi-synthetic diet containing
a very low level of fat (no fat (NF)) and six fat-supplemented diets (ca. 10%) based on barley and soybean meal. The
supplemental fat sources were tallow (T), high-oleic sunflower oil (HOSF), sunflower oil (SFO), linseed oil (LO), blend (FB) (55%
T, 35% SFO and 10% LO) and fish oil (FO) blend (40% FO and 60% LO). Pigs were slaughtered at 100 kg BW and autopsies
from liver, adipose tissue and muscle semimembranousus were collected for qPCR. The messenger ribonucleic acid (mRNA)
abundances of genes related to lipogenesis were modified due to dietary treatments in both liver (sterol regulatory elementbinding protein-1 (SREBP-1), acetyl CoA carboxylase (ACACA) and stearoyl CoA desaturase (SCD)) and adipose tissue (fatty acid
synthase (FASN), ACACA and SCD), but were not affected in semimembranousus muscle. In the liver, the mRNA abundances of
genes encoding lipogenic enzymes were highest in pigs fed HOSF and lowest in pigs fed FO. In adipose tissue, the mRNA
abundances were highest in pigs fed the NF diet and lowest in pigs fed T. The study demonstrated that dietary FAs stimulate
lipogenic enzyme gene expression differently in liver, fat and muscles tissues.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
636 - Animal husbandry and breeding in general. Livestock rearing. Breeding of domestic animals
Pages
8
Publisher
Elsevier
Is part of
Animal
Citation
Duran-Montgé, P., P. K. Theil, C. Lauridsen, and E. Esteve-Garcia. 2009. “Dietary Fat Source Affects Metabolism of Fatty Acids in Pigs as Evaluated by Altered Expression of Lipogenic Genes in Liver and Adipose Tissues.” Animal 3 (4): 535–542. doi: 10.1017/s1751731108003686
Grant agreement number
MEC/Programa nacional de recursos y tecnologías agroalimentarias/RTA03-060-C2-1/ES/Efecto del perfil en ácidos grasos de ración sobre el depósito y distribución de lípidos en el cerdo/
Program
Nutrició Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2692]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/