Viral shedding and environmental dispersion of two clade 2.3.4.4b H5 high pathogenicity avian infuenza viruses in experimentally infected mule ducks: implications for environmental sampling
Visualitza/Obre
Autor/a
Filaire, Fabien
Gaide, Nicolas
Valle, Rosa
Secula, Aurélie
Perlas, Albert
Foret‑Lucas, Charlotte
Cantero Portillo, Guillermo
Croville, Guillaume
Guerin, Jean‑Luc
Data de publicació
2024-08-12ISSN
0928-4249
Resum
High pathogenicity avian influenza viruses (HPAIVs) have caused major epizootics in recent years, with devastating consequences for poultry and wildlife worldwide. Domestic and wild ducks can be highly susceptible to HPAIVs, and infection leads to efficient viral replication and massive shedding (i.e., high titres for an extended time), contributing to widespread viral dissemination. Importantly, ducks are known to shed high amounts of virus in the earliest phase of infection, but the dynamics and impact of environmental contamination on the epidemiology of HPAIV outbreaks are poorly understood. In this study, we monitored mule ducks experimentally infected with two H5N8 clade 2.3.4.4b goose/Guangdong HPAIVs sampled in France in 2016–2017 and 2020–2021 epizootics. We investigated viral shedding dynamics in the oropharynx, cloaca, conjunctiva, and feathers; bird-to-bird viral transmission; and the role of the environment in viral spread and as a source of samples for early detection and surveillance. Our findings showed that viral shedding started before the onset of clinical signs, i.e., as early as 1 day post-inoculation (dpi) or post-contact exposure, peaked at 4 dpi, and lasted for up to 14 dpi. The detection of viral RNA in aerosols, dust, and water samples mirrored viral shedding dynamics, and viral isolation from these environmental samples was successful throughout the experiment. Our results confirm that mule ducks can shed high HPAIV titres through the four excretion routes tested (oropharyngeal, cloacal, conjunctival, and feather) while being asymptomatic and that environmental sampling could be a non-invasive tool for early viral RNA detection in HPAIV-infected farms.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
12
Publicat per
BMC
Publicat a
Veterinary Research
Citació
Filaire, Fabien, Kateri Bertran, Nicolas Gaide, Rosa Valle, Aurélie Secula, Albert Perlas, Charlotte Foret-Lucas, et al. 2024. “Viral Shedding and Environmental Dispersion of Two Clade 2.3.4.4b H5 High Pathogenicity Avian Influenza Viruses in Experimentally Infected Mule Ducks: Implications for Environmental Sampling.” Veterinary Research 55 (1). doi:10.1186/s13567-024-01357-z.
Número de l'acord de la subvenció
MICINN/Programa Estatal para Desarrollar, Atraer y Retener Talento/RYC-2021-033472-I/ES/La gripe aviar: patobiología y control/
EC/H2020/731014/EU/Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation/VetBioNet
Programa
Sanitat Animal
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