Culicoides Midge Bites Modulate the Host Response and Impact on Bluetongue Virus Infection in Sheep
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Author
Bréard, Emmanuel
Urien, Céline
Viarouge, Cyril
Lorca-Oro, Cristina
Jouneau, Luc
Charley, Bernard
Zientara, Stéphan
Solanes, David
Schwartz-Cornil, Isabelle
Publication date
2014-01-08ISSN
1932-6203
Abstract
Many haematophagous insects produce factors that help their blood meal and coincidently favor pathogen transmission. However nothing is known about the ability of Culicoides midges to interfere with the infectivity of the viruses they transmit. Among these, Bluetongue Virus (BTV) induces a hemorrhagic fever- type disease and its recent emergence in Europe had a major economical impact. We observed that needle inoculation of BTV8 in the site of uninfected C. nubeculosus feeding reduced viraemia and clinical disease intensity compared to plain needle inoculation. The sheep that developed the highest
local inflammatory reaction had the lowest viral load, suggesting that the inflammatory response to midge bites may participate in the individual sensitivity to BTV viraemia development. Conversely compared to needle inoculation, inoculation of BTV8 by infected C. nubeculosus bites promoted viraemia and clinical symptom expression, in association with delayed IFN- induced gene expression and retarded neutralizing antibody responses. The effects of uninfected and infected midge bites on BTV viraemia and on the host response indicate that BTV transmission by infected midges is the most reliable experimental method to study the physio-pathological events relevant to a natural infection and to pertinent vaccine evaluation in the target species. It also leads the way to identify the promoting viral infectivity factors of infected
Culicoides in order to possibly develop new control strategies against BTV and other Culicoides transmitted viruses.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
9
Publisher
Public Library of Science
Is part of
PLoS ONE
Citation
Pages, Nonito, Emmanuel Bréard, Céline Urien, Sandra Talavera, Cyril Viarouge, Cristina Lorca-Oro, and Luc Jouneau et al. 2014. "Culicoides Midge Bites Modulate The Host Response And Impact On Bluetongue Virus Infection In Sheep". Plos ONE 9 (1): e83683. doi:10.1371/journal.pone.0083683.
Grant agreement number
EC/FP7/228394/EU/Building an EU animal disease research network/NADIR
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2777]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/