Changes in Bacterial Population of Gastrointestinal Tract of Weaned Pigs Fed with Different Additives
View/Open
Author
Roca, Mercè
Castillo, Marisol
Martín-Orúe, Susana María
Espinal, Anna
Publication date
2014-01-19ISSN
2314-6133
Abstract
This study aimed to provide novel insights into the gastrointestinal microbial diversity from different gastrointestinal locations in weaning piglets using PCR-restriction fragment length polymorphism (PCR-RFLP). Additionally, the effect of different feed additives was analyzed. Thirty-two piglets were fed with four different diets: a control group and three enriched diets, with avilamycin, sodium butyrate, and a plant extract mixture. Digesta samples were collected from eight different gastrointestinal segments of each animal and the bacterial population was analysed by a PCR-RFLP technique that uses 16S rDNA gene sequences. Bacterial diversity was assessed by calculating the number of bands and the Shannon-Weaver index. Dendrograms were constructed to estimate the similarity of bacterial populations. A higher bacterial diversity was detected in large intestine compared to small intestine. Among diets, the most relevant microbial diversity differences were found between sodium butyrate and plant extract mixture. Proximal jejunum, ileum, and proximal colon were identified as those segments that could be representative of microbial diversity in pig gut. Results indicate that PCR-RFLP technique allowed detecting modifications on the gastrointestinal microbial ecology in pigs fed with different additives, such as increased biodiversity by sodium butyrate in feed.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
14
Publisher
Hindawi
Is part of
BioMed Research International
Citation
Roca, Mercè, Miquel Nofrarías, Natàlia Majó, Ana María Pérez De Rozas, Joaquim Segalés, Marisol Castillo, Susana María Martín-Orúe, Anna Espinal, Joan Pujols, and Ignacio Badiola. 2014. “Changes in Bacterial Population of Gastrointestinal Tract of Weaned Pigs Fed With Different Additives.” BioMed Research International 2014 (January): 1–13. https://doi.org/10.1155/2014/269402.
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2805]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/