Transcriptomic and proteomic analyses of a pale-green durum wheat mutant shows variations in photosystem components and metabolic deficiencies under drought stress
Author
Marè, Caterina
Aprile, Alessio
Roncaglia, Enrica
Cattivelli, Luigi
Publication date
2014-02-12ISSN
1471-2164
Abstract
Background: Leaf pigment content is an important trait involved in environmental interactions. In order to
determine its impact on drought tolerance in wheat, we characterized a pale-green durum wheat mutant
(Triticum turgidum L. var. durum) under contrasting water availability conditions. Results: The pale-green mutant was investigated by comparing pigment content and gene/protein expression profiles to wild-type plants at anthesis. Under well-watered (control) conditions the mutant had lower levels of chlorophylls and carotenoids, but higher levels of xanthophyll de-epoxidation compared to wild-type. Transcriptomic analysis under control conditions showed that defense genes (encoding e.g. pathogenesis-related proteins, peroxidases and chitinases) were upregulated in the mutant, suggesting the presence of mild oxidative stress that was compensated without altering the net rate of photosynthesis. Transcriptomic analysis under terminal water stress conditions, revealed the modulation of antioxidant enzymes, photosystem components, and enzymes representing carbohydrate metabolism and the tricarboxylic acid cycle, indicating that the mutant was exposed to greater oxidative stress than the wild-type plants, but had a limited capacity to respond. We also compared the two genotypes under irrigated and rain-fed field conditions over three years, finding that the greater oxidative stress and corresponding molecular changes in the pale-green mutant were associated to a yield reduction. Conclusions: This study provides insight on the effect of pigment content in the molecular response to drought.Identified genes differentially expressed under terminal water stress may be valuable for further studies addressing drought resistance in wheat.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
27
Publisher
BMC
Is part of
BMC Genomics
Citation
Peremarti, Ariadna, Caterina Marè, Alessio Aprile, Enrica Roncaglia, Luigi Cattivelli, Dolors Villegas, and Conxita Royo. 2014. "Transcriptomic And Proteomic Analyses Of A Pale-Green Durum Wheat Mutant Shows Variations In Photosystem Components And Metabolic Deficiencies Under Drought Stress". BMC Genomics 15 (1): 125. doi:10.1186/1471-2164-15-125.
Grant agreement number
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2009-11187/ES/Componentes De Tolerancia A La Sequia E Impacto En Mejora De Trigo: Bases Genetica Y Molecular Del Contenido De Chl/
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-37217/ES/HERRAMIENTAS PARA LA MEJORA DEL TRIGO DURO FRENTE A LOS RETOS DEL CAMBIO CLIMATICO/
INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2009-00085-00-00/ES/Bases fenológicas y genéticas de los patrones de adaptación del trigo duro a diversas condiciones fototérmicas y su utilidad para el desarrollo de germoplasma en el marco de la relación España/CIMMYT
Program
Cultius Extensius Sostenibles
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2845]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/