Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine
Visualitza/Obre
Autor/a
van der Poel, W.H.M.
Pavio, N.
van der Goot, J.
van Es, M.
Martin, M.
Engel, B.
Data de publicació
2014-03-21ISSN
1414-431X
Resum
Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 ELISA, a recombinant baculovirus expression product of HEV Gt3 open reading frame-2 was produced and coated onto polystyrene ELISA plates. After incubation of porcine sera, bound HEV antibodies were
detected with anti-porcine anti-IgG and anti-IgM conjugates. For primary estimation of sensitivity and specificity of the assay, sets of sera were used from pigs experimentally infected with HEV Gt3. For further validation of the assay and to set the cutoff value, a batch of 1100 pig sera was used. All pig sera were tested using the developed HEV Gt3 assay and two other serologic assays based on HEV Gt1 antigens. Since there is no gold standard available for HEV antibody testing, further validation and
a definite setting of the cutoff of the developed HEV Gt3 assay were performed using a statistical approach based on Bayes’ theorem. The developed and validated HEV antibody assay showed effective detection of HEV-specific antibodies. This assay can contribute to an improved detection of HEV antibodies and enable more reliable estimates of the prevalence of HEV Gt3 in swine in different regions.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
6
Publicat per
Associação Brasileira de Divulgação Científica
Publicat a
Brazilian Journal of Medical and Biological Research
Citació
van der Poel, W.H.M., N. Pavio, J. van der Goot, M. van Es, M. Martin, and B. Engel. 2014. “Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine”. Brazilian Journal of Medical and Biological Research 47 (4): 334-339. doi:10.1590/1414-431X20133249.
Número de l'acord de la subvenció
EC/FP6/506122/EU/Network for prevention and control of zoonoses/MED-VET-NET
Programa
Sanitat Animal
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