Development of a PCR‐based method to monitor arthropod dispersal in agroecosystems: Macrolophus pygmaeus (Hemiptera: Miridae) from banker plants to tomato crops
View/Open
Publication date
2019-08-16ISSN
1672-9609
Abstract
Development of conservation biological control programs requires the identification of sources that contribute to predator colonization of crops. Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae) is an efficient polyphagous predator used in biological control programs in vegetable crops in Europe. We have developed a marking method based on spraying with a solution of the brine shrimp Artemia spp. (Anostraca: Artemiidae) cysts, followed by a PCR detection of Artemia DNA to monitor M. pygmaeus dispersal from banker plants to tomato crops. Experiments conducted in climatic chambers show that the topical application of this marking solution on M. pygmaeus does not significantly reduce adult longevity and that it is detected up to 6 d after the application. When this Artemia solution was applied on Calendula officinalis L. banker plants harboring M. pygmaeus and maintained outdoors, Artemia DNA was still detected on 62% of the insects after 6 d. The conducted field applications in commercial greenhouses have confirmed the usefulness of this method to monitor M. pygmaeus dispersal from banker plants to a newly planted tomato crop. This method can be used to assess arthropod movement, being an interesting molecular approach for further improving future pest management strategies.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
632 - Plant damage, injuries. Plant diseases. Pests, organisms injurious to plants. Plant protection
Pages
24
Publisher
Wiley
Is part of
Insect Science
Citation
Agustí, Nuria, Cristina Castañé, Irene Fraile, and Oscar Alomar. 2020. "Development Of A PCR‐Based Method To Monitor Arthropod Dispersal In Agroecosystems: Macrolophus Pygmaeus (Hemiptera: Miridae) From Banker Plants To Tomato Crops". Insect Science. Wiley. doi:10.1111/1744-7917.12717.
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-53970-C2-2-R/ES/EL PAISAJE EN EL CONTROL BIOLOGICO POR CONSERVACION EN FRUTALES DE HUESO/LANDFRUITBIOCON
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2011‐24349/ES/Identificación y evaluación de depredadores y sus fuentes de colonización para favorecer el control biológico por conservación en cultivos hortícolas/
Program
Protecció Vegetal Sostenible
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2850]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/