Diat.barcode, an open-access curated barcode library for diatoms
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Author
Rimet, Frédéric
Gusev, Evgenuy
Kahlert, Maria
Kelly, Martyn G.
Kulikovskiy, Maxim
Maltsev, Yevhen
Mann, David G.
Pfannkuchen, Martin
Vasselon, Valentin
Zimmermann, Jonas
Bouchez, Agnès
Publication date
2019-10-22ISSN
2045-2322
Abstract
Diatoms (Bacillariophyta) are ubiquitous microalgae which produce a siliceous exoskeleton and which make a major contribution to the productivity of oceans and freshwaters. They display a huge diversity, which makes them excellent ecological indicators of aquatic ecosystems. Usually, diatoms are identified using characteristics of their exoskeleton morphology. DNA-barcoding is an alternative to this and the use of High-Throughput-Sequencing enables the rapid analysis of many environmental samples at a lower cost than analyses under microscope. However, to identify environmental sequences correctly, an expertly curated reference library is needed. Several curated libraries for protists exists; none, however are dedicated to diatoms. Diat.barcode is an open-access library dedicated to diatoms which has been maintained since 2012. Data come from two sources (1) the NCBI nucleotide database and (2) unpublished sequencing data of culture collections. Since 2017, several experts have collaborated to curate this library for rbcL, a chloroplast marker suitable for species-level identification of diatoms. For the latest version of the database (version 7), 605 of the 3482 taxonomical names originally assigned by the authors of the rbcL sequences were modified after curation. The database is accessible at https://www6.inra.fr/carrtel-collection_eng/Barcoding-database.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
12
Publisher
Nature Research
Is part of
Scientific Reports
Citation
Rimet, Frédéric, Evgenuy Gusev, Maria Kahlert, Martyn G. Kelly, Maxim Kulikovskiy, Yevhen Maltsev, and David G. Mann et al. 2019. "Diat.Barcode, An Open-Access Curated Barcode Library For Diatoms". Scientific Reports 9 (1). Springer Science and Business Media LLC. doi:10.1038/s41598-019-51500-6.
Grant agreement number
EC/COST/CA15219/EU/Developing new genetic tools for bioassessment of aquatic ecosystems in Europe/DNAqua-Net
Program
Aigües Marines i Continentals
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2831]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/