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dc.contributor.authorCriado-Mesas, Lourdes
dc.contributor.authorBallester, Maria
dc.contributor.authorCrespo-Piazuelo, Daniel
dc.contributor.authorCastelló, Anna
dc.contributor.authorBenítez, Rita
dc.contributor.authorFernández, Ana Isabel
dc.contributor.authorFolch, Josep M.
dc.contributor.otherProducció Animalca
dc.date.accessioned2019-12-05T09:07:53Z
dc.date.available2019-12-05T09:07:53Z
dc.date.issued2019-08-08
dc.identifier.citationCriado-Mesas, Lourdes, Maria Ballester, Daniel Crespo-Piazuelo, Anna Castelló, Rita Benítez, Ana Isabel Fernández, and Josep M. Folch. 2019. "Analysis Of Porcine IGF2 Gene Expression In Adipose Tissue And Its Effect On Fatty Acid Composition". PLOS ONE 14 (8): e0220708. Public Library of Science (PLoS). doi:10.1371/journal.pone.0220708.ca
dc.identifier.issn1932-6203ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/577
dc.description.abstractIGF2:g.3072G>A polymorphism has been described as the causal mutation of a maternally imprinted QTL for muscle growth and fat deposition in pigs. The objective of the current work was to study the association between the IGF2:g.3072G>A polymorphism and the IGF2 gene expression and its effect on fatty acid composition in adipose tissue in different pig genetic backgrounds. A cis-eQTL region associated with the IGF2 mRNA expression in adipose tissue was identified in an eGWAS with 355 animals. The IGF2 gene was located in this genomic interval and IGF2g.3072G>A was the most significant SNP, explaining a 25% of the gene expression variance. Significant associations between IGF2:g.3072G>A polymorphism and oleic (C18:1(n-9); p-value = 4.18x10-07), hexadecanoic (C16:1(n-9); p-value = 4.04x10-07), linoleic (C18:2(n-6); p-value = 6.44x10-09), α-linoleic (C18:3(n-3); p-value = 3.30x10-06), arachidonic (C20:4(n-6); p-value = 9.82x10-08) FAs and the MUFA/PUFA ratio (p-value = 2.51x10-9) measured in backfat were identified. Animals carrying the A allele showed an increase in IGF2 gene expression and higher PUFA and lower MUFA content. However, in additional studies was observed that there could be other proximal genetic variants affecting FA composition in adipose tissue. Finally, no differences in the IGF2 gene expression in adipose tissue were found between heterozygous animals classified according to the IGF2:g.3072G>A allele inherited from the father (APGM or AMGP). However, pyrosequencing analysis revealed that there is imprinting of the IGF2 gene in muscle and adipose tissues, with stronger differences among the paternally and maternally inherited alleles in muscle. Our results suggested that IGF2:g.3072G>A polymorphism plays an important role in the regulation of IGF2 gene expression and can be involved in the fatty acid composition in adipose tissue. In both cases, further studies are still needed to deepen the mechanism of regulation of IGF2 gene expression in adipose tissue and the IGF2 role in FA composition.ca
dc.format.extent18ca
dc.language.isoengca
dc.publisherPublic Library of Scienceca
dc.relation.ispartofPlos Oneca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleAnalysis of porcine IGF2 gene expression in adipose tissue and its effect on fatty acid compositionca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO-FEDER/Programa estatal de I+D+I orientada a los retos de la sociedad/AGL2014-56369-C2-2-R/ES//Validación genética y funcional de QTLS, genes y redes génicas y estudio del efecto del microbioma sobre crecimiento, deposición de grasa y calidad de carne en porcino. S2/ca
dc.relation.projectIDMINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-82641-R/ES/Genómica funcional, biología de sistemas y microbiomica aplicadas a la identificación de reguladores genéticos del crecimiento, engrasamiento y calidad de la carne en porcino/ca
dc.relation.projectIDMINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC‑2013‑12573/ES/ /ca
dc.subject.udc636ca
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0220708ca
dc.contributor.groupGenètica i Millora Animalca


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