A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs
Autor/a
In-Cheol, Cho
Hee-Bok, Park
Jin Seop, Ahn
Sang-Hyun, Han
Jae-Bong, Lee
Hyun-Tae, Lim
Chae-Kyoung, Yoo
Eun-Ji, Jung
Dong-Hwan, Kim
Wu-Sheng, Sun
Sang-Geum, Kim
Yong-Jun, Kang
Yoo-Kyung, Kim
Hyun-Sook, Shin
Pil-Nam, Seong
In-Sul, Hwang
Beom-Young, Park
Seongsoo, Hwang
Sung-Soo, Lee
Youn-Chul, Ryu
Jun-Heon, Lee
Moon-Suck, Ko
Kichoon, Lee
Andersson, Göran
Pérez-Enciso, Miguel
Jeong-Jeong, Lee
Fecha de publicación
2019-10-11ISSN
1553-7390
Resumen
Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5′-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
663/664 - Alimentos y nutrición. Enología. Aceites. Grasas
Páginas
26
Publicado por
Public Library of Science
Publicado en
PLoS Genetics
Citación
Cho, In-Cheol, Hee-Bok Park, Jin Seop Ahn, Sang-Hyun Han, Jae-Bong Lee, Hyun-Tae Lim, and Chae-Kyoung Yoo et al. 2019. "A Functional Regulatory Variant Of MYH3 Influences Muscle Fiber-Type Composition And Intramuscular Fat Content In Pigs". PLOS Genetics 15 (10): e1008279. Public Library of Science (PLoS). doi:10.1371/journal.pgen.1008279.
Número del acuerdo de la subvención
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-78709-R/ES/UTILIZACION DE SECUENCIAS COMPLETAS PARA LA MEJORA DE ESPECIES DOMESTICAS/
Program
Genètica i Millora Animal
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