Efficacy of Two Licensed Avian Influenza H5 Vaccines Against Challenge with a 2015 U.S. H5N2 clade 18.104.22.168 Highly Pathogenic Avian Influenza Virus in Domestic Ducks
Pantin-Jackwood, Mary J.
Kapczynski, Darrell R.
Suarez, David L.
Highly pathogenic avian influenza (HPAI) clade 22.214.171.124 viruses from the H5 goose/Guangdong lineage caused a major outbreak in poultry in the United States in 2015. Although the outbreak was controlled, vaccines were considered as an alternative control method, and new vaccines were approved and purchased by the U.S. Department of Agriculture National Veterinary Stockpile for emergency use. In this study, we evaluated the efficacy of two of these vaccines in protecting Pekin ducks (Anas platyrhynchos var. domestica) against challenge with a H5N2 HPAI poultry isolate. A recombinant alphavirus-based vaccine and an inactivated adjuvanted reverse genetics vaccine, both expressing the hemagglutinin gene of a U.S. H5 clade 126.96.36.199 isolate (A/Gyrfalcon/Washington/41088-6/2014 H5N8), were used to immunize the ducks. The vaccines were given either as single vaccination at 2 days of age or in a prime-boost strategy at 2 and 15 days of age. At 32 days of age, all ducks were challenged with A/turkey/Minnesota/12582/15 H5N2 HPAI virus clade 188.8.131.52. All ducks from the nonvaccinated challenge control group became infected and shed virus; one duck in this group presented mild ataxia, and a second duck died. No mortality or clinical signs were observed in vaccinated and challenged ducks, with the exception of one duck presenting with mild ataxia. Both vaccines, regardless of the vaccination strategy used, were immunogenic in ducks and reduced or prevented virus shedding after challenge. In conclusion, good protection against H5Nx infection was achieved in ducks vaccinated with the vaccines examined, which were homologous to the challenge virus, with prime-boost strategies conferring the best protection against infection.
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619 - Veterinaria
American Association of Avian Pathologists
Mary J. Pantin-Jackwood , Eric De Jesús , Mar Costa-Hurtado , Diane Smith , Klaudia Chrząstek , Darrell R. Kapczynski , y David L. Suárez "Eficacia de dos Licensed la gripe aviar H5 Las vacunas contra el desafío con un 2015 por US H5N2 clado 2.3. 4.4 Virus de la influenza aviar altamente patógena en patos domésticos, " Avian Diseases 63 (1), 90-96, (6 de agosto de 2018). https://doi.org/10.1637/11895-050918-Reg.1
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