Differential distribution of the wlaN and cgtB genes, Associated with Guillain-Barré Syndrome, in Campylobacter jejuni isolates from humans, broiler chickens, and wild birds
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Author
Guirado, Pedro
Paytubi, Sonia
Miró, Elisenda
Iglesias-Torrens, Yaidelis
Navarro, Ferran
Attolini, Camille Stephan-Otto
Balsalobre, Carlos
Madrid, Cristina
Publication date
2020-02-26ISSN
2076-2607
Abstract
Campylobacter jejuni causes campylobacteriosis, a bacterial gastroenteritis with high
incidence worldwide. Moreover, C. jejuni infection can trigger the polyneuropathic disorder
denominated Guillain-Barré syndrome (GBS). The C. jejuni strains that can elicit GBS carry either
wlaN or cgtB, coding both genes for a β-1,3-galactosyltransferase enzyme that is required for the
production of sialylated lipooligosaccharide (LOSSIAL). We described a differential prevalence of the
genes wlaN and cgtB in C. jejuni isolates from three different ecological niches: humans, broiler
chickens, and wild birds. The distribution of both genes, which is similar between broiler chicken
and human isolates and distinct when compared to the wild bird isolates, suggests a host-dependent
distribution. Moreover, the prevalence of the wlaN and cgtB genes seems to be restricted to some
clonal complexes. Gene sequencing identified the presence of new variants of the G- homopolymeric
tract within the wlaN gene. Furthermore, we detected two variants of a G rich region within the cgtB
gene, suggesting that, similarly to wlaN, the G-tract in the cgtB gene mediates the phase variation
control of cgtB expression. Caco-2 cell invasion assays indicate that there is no evident correlation
between the production of LOSSIAL and the ability to invade eukaryotic cells.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
12
Publisher
MDPI
Is part of
Microorganisms
Citation
Guirado, Pedro, Sonia Paytubi, Elisenda Miró, Yaidelis Iglesias-Torrens, Ferran Navarro, Marta Cerdà-Cuéllar, Camille Stephan-Otto Attolini, Carlos Balsalobre, and Cristina Madrid. 2020. "Differential Distribution Of The Wlan And Cgtb Genes, Associated With Guillain-Barré Syndrome, In Campylobacter Jejuni Isolates From Humans, Broiler Chickens, And Wild Birds". Microorganisms 8 (3): 325. MDPI AG. doi:10.3390/microorganisms8030325.
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-45339R/ES/Identificación de factores implicados en la transmisión de la campilobacteriosis por alimentos/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2694]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/