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dc.contributor.authorSaporiti, Viviane
dc.contributor.authorHuerta, Eva
dc.contributor.authorCorrea-Fiz, Florencia
dc.contributor.authorGrosse Liesner, Bernd
dc.contributor.authorDuran, Oliver
dc.contributor.authorSegalés, Joaquim
dc.contributor.authorSibila, Marina
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-05-18T09:10:55Z
dc.date.available2020-05-18T09:10:55Z
dc.date.issued2020-05-01
dc.identifier.citationSaporiti, Viviane, Eva Huerta, Florencia Correa‐Fiz, Bernd Grosse Liesner, Oliver Duran, Joaquim Segalés, and Marina Sibila. 2020. "Detection And Genotyping Of Porcine Circovirus 2 (PCV‐2) And Detection Of Porcine Circovirus 3 (PCV‐3) In Sera From Fattening Pigs Of Different European Countries". Transboundary And Emerging Diseases. Wiley. doi:10.1111/tbed.13596.ca
dc.identifier.issn1865-1674ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/794
dc.description.abstractPCV-2 is considered one of the most economically important viral agents in swine worldwide. Recently, PCV-3 has been found in pigs affected by different disorders and in healthy animals. The objective of this epidemiological work was to describe the frequency of detection of PCV-2 and PCV-3 in pig farms of 9 European countries. Moreover, a second aim was to assess the most frequent PCV-2 genotypes found in the studied farms. Sera from 5 to 10 pigs per farm were collected from 2 to 11 farms per studied country. A total of 624 sera of fattening pigs (10-25 week old) from 64 farms from Spain (n = 11), Belgium (n = 10), France (n = 8), Germany (n = 8), Italy (n = 7), Denmark (n = 8), the Netherlands (n = 5), Ireland (n = 5) and Sweden (n = 2) were analysed by conventional PCR. In addition, one or two PCV-2-positive samples per farm were genotyped by sequencing the ORF2 gene. PCV-3 PCR-positive samples with relatively low Ct values were also sequenced and phylogenetically analysed. PCV-2 DNA was detected in pig sera from all European tested countries, but Sweden. A total of 132 out of 624 (21%) sera were positive for PCV-2 PCR, corresponding to 30 out of the 64 (47%) tested farms. PCV-3 DNA was detected in 52 out of 624 (8%) sera, corresponding also to 30 out of the 64 (47%) studied farms from all tested countries. A total of 48 PCV-2 PCR-positive samples were successfully sequenced and genotyped, being PCV-2d the most frequently genotype found (n = 28), followed by PCV-2b (n = 11) and PCV-2a (n = 9). These results pointed out PCV-2d as the most prevalent genotype currently in Europe. The PCV-3 phylogenetic analysis showed high identity (>98%) among sequences from all the analysed countries. The relatively low co-infection (3%), likely suggest an independent circulation patterns of PCV-2 and PCV-3.ca
dc.format.extent11ca
dc.language.isoengca
dc.publisherWileyca
dc.relation.ispartofTransboundary and Emerging Diseasesca
dc.rightsAttribution-NonCommercial 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.titleDetection and genotyping of Porcine circovirus 2 (PCV‐2) and detection of Porcine circovirus 3 (PCV‐3) in sera from fattening pigs of different European countriesca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa estatal de I+D+I orientada a los retos de la sociedad/E-RTA2017-00007-00-00/ES/Impacto de la infección por un nuevo circovirus porcino (PCV3) en España: desarrollo de herramientas diagnósticas, asociación con enfermedad y exploración de métodos de prevención y control/ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1111/tbed.13596ca
dc.contributor.groupSanitat Animalca


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