Short communication: Recombinant mammary serum amyloid A3 as a potential strategy for preventing intramammary infections in dairy cows at dryoff
Mammary serum amyloid A3 (M-SAA3) has shown potential in stimulating innate immunity during intramammary infections, at calving and at dryoff. In this study, we produced recombinant caprine M-SAA3 to test its ability to reduce intramammary infections with Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, and Escherichia coli, which are all common mastitis-producing pathogens. Recombinant production of M-SAA3 (followed by lipopolysaccharide removal to avoid lipopolysaccharide-nonspecific stimulation of the immune system) was successfully achieved. Mammary serum amyloid A3 stimulated the expression of IL-8 in a dose-dependent manner in primary mammary cultures. Although a direct killing effect on Staph. aureus by M-SAA3 was not detected, this acute phase protein was able to reduce Staph. aureus, Strep. uberis, and Strep. dysgalactiae infections by up to 50% and induced a reduction in E. coli counts of 67%. In general, the best concentration of caprine M-SAA3 for inhibiting infections was the lowest concentration tested (10 μg/mL), although higher concentrations (up to 160 μg/mL) increased its antimicrobial potential against some pathogens.
63 - Agriculture and related sciences and techniques
American Dairy Science Association
Is part of
Journal of Dairy Science
Parés, Sílvia, Francesc Fàbregas, Àlex Bach, Elena Garcia-Fruitós, Ana de Prado, and Anna Arís. 2020. "Short Communication: Recombinant Mammary Serum Amyloid A3 As A Potential Strategy For Preventing Intramammary Infections In Dairy Cows At Dryoff". Journal Of Dairy Science 103 (4): 3615-3621. doi:10.3168/jds.2019-17276.
Grant agreement number
INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00028-C02-02/ES/Optimización del periodo de secado de la vaca mediante nanopartículas proteicas/
INIA/Programa Estatal de I+D+I orientada a los retos de la Sociedad/RTA2015-00064-C02/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/
Producció de Remugants
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- ARTICLES CIENTÍFICS 
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