dc.contributor.author | Parés, Sílvia | |
dc.contributor.author | Fàbregas, Francesc | |
dc.contributor.author | Bach, Àlex | |
dc.contributor.author | Garcia-Fruitós, Elena | |
dc.contributor.author | de Prado, Ana | |
dc.contributor.author | Arís, Anna | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2020-07-27T09:13:02Z | |
dc.date.available | 2022-03-24T12:00:21Z | |
dc.date.issued | 2020-02-11 | |
dc.identifier.citation | Parés, Sílvia, Francesc Fàbregas, Àlex Bach, Elena Garcia-Fruitós, Ana de Prado, and Anna Arís. 2020. "Short Communication: Recombinant Mammary Serum Amyloid A3 As A Potential Strategy For Preventing Intramammary Infections In Dairy Cows At Dryoff". Journal Of Dairy Science 103 (4): 3615-3621. doi:10.3168/jds.2019-17276. | ca |
dc.identifier.issn | 0022-0302 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/876 | |
dc.description.abstract | Mammary serum amyloid A3 (M-SAA3) has shown potential in stimulating innate immunity during intramammary infections, at calving and at dryoff. In this study, we produced recombinant caprine M-SAA3 to test its ability to reduce intramammary infections with Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, and Escherichia coli, which are all common mastitis-producing pathogens. Recombinant production of M-SAA3 (followed by lipopolysaccharide removal to avoid lipopolysaccharide-nonspecific stimulation of the immune system) was successfully achieved. Mammary serum amyloid A3 stimulated the expression of IL-8 in a dose-dependent manner in primary mammary cultures. Although a direct killing effect on Staph. aureus by M-SAA3 was not detected, this acute phase protein was able to reduce Staph. aureus, Strep. uberis, and Strep. dysgalactiae infections by up to 50% and induced a reduction in E. coli counts of 67%. In general, the best concentration of caprine M-SAA3 for inhibiting infections was the lowest concentration tested (10 μg/mL), although higher concentrations (up to 160 μg/mL) increased its antimicrobial potential against some pathogens. | ca |
dc.format.extent | 21 | ca |
dc.language.iso | eng | ca |
dc.publisher | American Dairy Science Association | ca |
dc.relation.ispartof | Journal of Dairy Science | ca |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Short communication: Recombinant mammary serum amyloid A3 as a potential strategy for preventing intramammary infections in dairy cows at dryoff | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.relation.projectID | INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00028-C02-02/ES/Optimización del periodo de secado de la vaca mediante nanopartículas proteicas/ | ca |
dc.relation.projectID | INIA/Programa Estatal de I+D+I orientada a los retos de la Sociedad/RTA2015-00064-C02/ES/Validación del uso de las proteínas M-SAA3 y MMP-9 en la mejora del secado de la vaca de leche y optimización de su dosis efectiva mediante su nanoestructuración/ | ca |
dc.subject.udc | 63 | ca |
dc.identifier.doi | https://doi.org/10.3168/jds.2019-17276 | ca |
dc.contributor.group | Producció de Remugants | ca |