Identification and characterization of Diaporthe spp. associated with twig cankers and shoot blight of almonds in Spain
Rodríguez-Reina, José M.
Arroyo, Francisco T.
Two hundred and twenty-five Diaporthe isolates were collected from 2005 to 2019 in almond orchards showing twig cankers and shoot blight symptoms in five different regions across Spain. Multilocus DNA sequence analysis with five loci (ITS, tub, tef-1α, cal and his), allowed the identification of four known Diaporthe species, namely: D. amygdali, D. eres, D. foeniculina and D. phaseolorum. Moreover, a novel phylogenetic species, D. mediterranea, was described. Diaporthe amygdali was the most prevalent species, due to the largest number of isolates (85.3%) obtained from all sampled regions. The second most frequent species was D. foeniculina (10.2%), followed by D. mediterranea (3.6%), D. eres and D. phaseolorum, each with only one isolate. Pathogenicity tests were performed using one-year-old almond twigs cv. Vayro and representative isolates of the different species. Except for D. foeniculina and D. phaseolorum, all Diaporthe species were able to cause lesions significantly different from those developed on the uninoculated controls. Diaporthe mediterranea caused the most severe symptoms. These results confirm D. amygdali as a key pathogen of almonds in Spain. Moreover, the new species, D. mediterranea, should also be considered as a potential important causal agent of twig cankers and shoot blight on this crop
632 - Malalties i protecció de les plantes
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León, Maela, Mónica Berbegal, José M. Rodríguez-Reina, Georgina Elena, Paloma Abad-Campos, Antonio Ramón-Albalat, and Diego Olmo et al. 2020. "Identification And Characterization Of Diaporthe Spp. Associated With Twig Cankers And Shoot Blight Of Almonds In Spain". Agronomy 10 (8): 1062. doi:10.3390/agronomy10081062.
Grant agreement number
MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad en I+D+I/RYC-2017-23098/ES/ /
INIA-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2017-00009-C04-01/ES/Estrategias de control de enfermedades fúngicas del almendro basadas en la epidemiología, en la genética de la resistencia y en las prácticas de cultivo/
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