dc.contributor.author | Wang, Lihua | |
dc.contributor.author | Mi, Shijiang | |
dc.contributor.author | Madera, Rachel | |
dc.contributor.author | Ganges, Llilianne | |
dc.contributor.author | Borca, Manuel V. | |
dc.contributor.author | Ren, Jingqiang | |
dc.contributor.author | Cunningham, Chase | |
dc.contributor.author | Cino-Ozuna, Ada G. | |
dc.contributor.author | Li, Hongwei | |
dc.contributor.author | Tu, Changchun | |
dc.contributor.author | Gong, Wenjie | |
dc.contributor.author | Shi, Jishu | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2020-08-03T08:41:41Z | |
dc.date.available | 2020-08-03T08:41:41Z | |
dc.date.issued | 2020-01-14 | |
dc.identifier.citation | Wang, Lihua, Shijiang Mi, Rachel Madera, Llilianne Ganges, Manuel V. Borca, Jingqiang Ren, and Chase Cunningham et al. 2020. "A Neutralizing Monoclonal Antibody-Based Competitive ELISA For Classical Swine Fever C-Strain Post–Vaccination Monitoring". BMC Veterinary Research 16 (1). doi:10.1186/s12917-020-2237-6. | ca |
dc.identifier.issn | 1746-6148 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/884 | |
dc.description.abstract | Background: Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation of CSF vaccines. However, VNT is a time consuming procedure that requires cell culture and live virus manipulation. C-strain CSF vaccine is the most frequently used vaccine for CSF control and prevention. In this study, we presented a neutralizing monoclonal antibody (mAb) based competitive enzyme-linked immunosorbent assay (cELISA) with the emphasis on the replacement of VNT for C-strain post–vaccination monitoring. Results: One monoclonal antibody (6B211) which has potent neutralizing activity against C-strain was generated. A novel cELISA was established and optimized based on the strategy that 6B211 can compete with C-strain induced neutralizing antibodies in pig serum to bind capture antigen C-strain E2. By testing C-strain VNT negative pig sera (n = 445) and C-strain VNT positive pig sera (n = 70), the 6B211 based cELISA showed 100% sensitivity (95% confidence interval: 94.87 to 100%) and 100% specificity (95% confidence interval: 100 to 100%). The C-strain antibody can be tested in pigs as early as 7 days post vaccination with the cELISA. By testing pig sera (n = 139) in parallel, the cELISA showed excellent agreement (Kappa = 0.957) with VNT. The inhibition rate of serum samples in the cELISA is highly correlated with their titers in VNT (r2 = 0.903, p < 0.001). In addition, intra- and inter-assays of the cELISA exhibited acceptable repeatability with low coefficient of variations (CVs). Conclusions: This novel cELISA demonstrated excellent agreement and high level correlation with VNT. It is a reliable tool for sero-monitoring of C-strain vaccination campaign because it is a rapid, simple, safe and cost effective assay that can be used to monitor vaccination-induced immune response at the population level. | ca |
dc.format.extent | 8 | ca |
dc.language.iso | eng | ca |
dc.publisher | BMC | ca |
dc.relation.ispartof | BMC Veterinary Research | ca |
dc.rights | Attribution 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post–vaccination monitoring | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.1186/s12917-020-2237-6 | ca |
dc.contributor.group | Sanitat Animal | ca |