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dc.contributor.authorWang, Lihua
dc.contributor.authorMi, Shijiang
dc.contributor.authorMadera, Rachel
dc.contributor.authorGanges, Llilianne
dc.contributor.authorBorca, Manuel V.
dc.contributor.authorRen, Jingqiang
dc.contributor.authorCunningham, Chase
dc.contributor.authorCino-Ozuna, Ada G.
dc.contributor.authorLi, Hongwei
dc.contributor.authorTu, Changchun
dc.contributor.authorGong, Wenjie
dc.contributor.authorShi, Jishu
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-08-03T08:41:41Z
dc.date.available2020-08-03T08:41:41Z
dc.date.issued2020-01-14
dc.identifier.citationWang, Lihua, Shijiang Mi, Rachel Madera, Llilianne Ganges, Manuel V. Borca, Jingqiang Ren, and Chase Cunningham et al. 2020. "A Neutralizing Monoclonal Antibody-Based Competitive ELISA For Classical Swine Fever C-Strain Post–Vaccination Monitoring". BMC Veterinary Research 16 (1). doi:10.1186/s12917-020-2237-6.ca
dc.identifier.issn1746-6148ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/884
dc.description.abstractBackground: Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation of CSF vaccines. However, VNT is a time consuming procedure that requires cell culture and live virus manipulation. C-strain CSF vaccine is the most frequently used vaccine for CSF control and prevention. In this study, we presented a neutralizing monoclonal antibody (mAb) based competitive enzyme-linked immunosorbent assay (cELISA) with the emphasis on the replacement of VNT for C-strain post–vaccination monitoring. Results: One monoclonal antibody (6B211) which has potent neutralizing activity against C-strain was generated. A novel cELISA was established and optimized based on the strategy that 6B211 can compete with C-strain induced neutralizing antibodies in pig serum to bind capture antigen C-strain E2. By testing C-strain VNT negative pig sera (n = 445) and C-strain VNT positive pig sera (n = 70), the 6B211 based cELISA showed 100% sensitivity (95% confidence interval: 94.87 to 100%) and 100% specificity (95% confidence interval: 100 to 100%). The C-strain antibody can be tested in pigs as early as 7 days post vaccination with the cELISA. By testing pig sera (n = 139) in parallel, the cELISA showed excellent agreement (Kappa = 0.957) with VNT. The inhibition rate of serum samples in the cELISA is highly correlated with their titers in VNT (r2 = 0.903, p < 0.001). In addition, intra- and inter-assays of the cELISA exhibited acceptable repeatability with low coefficient of variations (CVs). Conclusions: This novel cELISA demonstrated excellent agreement and high level correlation with VNT. It is a reliable tool for sero-monitoring of C-strain vaccination campaign because it is a rapid, simple, safe and cost effective assay that can be used to monitor vaccination-induced immune response at the population level.ca
dc.format.extent8ca
dc.language.isoengca
dc.publisherBMCca
dc.relation.ispartofBMC Veterinary Researchca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post–vaccination monitoringca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1186/s12917-020-2237-6ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/