Resynthesis: Marker-Based Partial Reconstruction of Elite Genotypes in Clonally-Reproducing Plant Species
Alexiou, Konstantinos G.
We propose a method for marker-based selection of cultivars of clonally-reproducing plant species which keeps the basic genetic architecture of a top-performing cultivar (usually a partly heterozygous genotype), with the addition of some agronomically relevant differences (such as production time, product appearance or quality), providing added value to the product or cultivation process. The method is based on selecting a) two complementary nearly-inbred lines from successive selfing generations (ideally only F2 and F3) of large size, that may generate individuals with most of their genome identical to the original cultivar but being homozygous for either of the two component haplotypes in the rest, and b) individuals with such characteristics already occurring in the F2. Option a) allows for introgressing genes from other individuals in one or both of these nearly-inbred lines. Peach, a woody-perennial, clonally-reproduced species, was chosen as a model for a proof of concept of the Resynthesis process due to its biological characteristics: self-compatibility, compact and genetically well-known genome, low recombination rates and relatively short intergeneration time (3–4 years). From 416 F2 seedlings from cultivar Sweet Dream (SD), we obtained seven individuals with 76–94% identity with SD, and selected five pairs of complementary lines with average homozygosity of the two parents ≥0.70 such that crossing would produce some individuals highly similar to SD. The application of this scheme to other species with more complex genomes or biological features, including its generalization to F1 hybrids, is discussed.
633 - Cultius i produccions
Is part of
Frontiers in Plant Science
Eduardo, Iban, Simó Alegre, Konstantinos G. Alexiou, and Pere Arús. 2020. "Resynthesis: Marker-Based Partial Reconstruction Of Elite Genotypes In Clonally-Reproducing Plant Species". doi:10.1101/2020.05.09.085738.
Grant agreement number
MINECO-FEDER/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-40228-C02-01/ES/USO DE LA SECUENCIA GENOMICA PARA LA CARACTERIZACION DE LA VARIABILIDAD INTRACLONAL E INTERESPECIFICA EN MELOCOTONERO Y ALMENDRO/
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00050-00-00/ES/Nuevas estrategias de mejora del melocotonero basadas en marcadores moleculares: introgresión asistida por marcadores y resíntesis/
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
Genòmica i Biotecnologia
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- ARTICLES CIENTÍFICS 
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